Words similar to ifngr
Example sentences for: ifngr
How can you use “ifngr” in a sentence? Here are some example sentences to help you improve your vocabulary:
interferon-gamma receptor IFNgR
Staining of fresh and trypsin-treated monocytes demonstrated that epitopes recognized by the mAb's against MHCII, IFNgR1 and IFNgR2 were resistant to 30 min digestion with 0.25% trypsin.
interferon-gamma receptor beta-chain IFNgR2
Another reported mechanism of IFNg resistance in tumor cells is associated with down-regulation of IFNg-receptors [ 38 ] . The relevance of this mechanism to MHCII inducibility was recently supported by the evidence that PMA is able to enhance IFNg-dependent MHCII expression in THP-1 human leukemia cells through the up-regulation of IFNg receptors [ 19 ] . In our experiments, however, the incubation of LS1034 cells with PMA and ethanol did not lead to any changes in IFNgR expression as determined by flow cytometry (Figure 4).
Cytoplasmic IFNgR1 and IFNgR2 receptor subunits were detected by using a procedure described for intracellular cytokine staining [ 50 ] . Briefly, cells were fixed in ice-cold 4% formaldehyde for 5 min, washed 2 times, permeabilized in staining buffer containing 0.2% saponin for 60 min at 4°C, incubated with biotin-conjugated mAb's (specific or isotype-matched) for 30 min, washed 2 times, incubated with SA-PE and washed again (saponin was present in staining buffer at all times).