Words similar to ifka

Example sentences for: ifka

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Attempts to disrupt ifkA were carried out using a plasmid that contained a blasticidin resistance cassette flanked by two fragments of the ifkA gene, each about 800 bp long and derived from the pseudokinase and kinase domains.
The predicted amino acid sequence of IfkA has all the hallmarks of being a kinase for eIF2α.
Identification of the ifk genes and disruption of the ifkA gene
GCN2 of yeast and mammals is a known sensor of amino acid starvation and regulates cellular responses to such starvation [ 24 25 ] . Using degenerate primers and PCR and database searching identified two GCN2-like genes in Dictyostelium , ifkA and ifkB . Thinking that one or both of these genes may play a role in sensing amino acid starvation and in initiating development, we carried out the work described herein to examine their functions.
This fragment was transformed into exponentially growing Ax4 cells using electroporation [ 49 ] . Transformants were selected as described [ 50 ] . Clonal isolates were obtained, and disruption of the ifkA gene was confirmed by PCR and Southern analysis.