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The cDNA was made radioactive by incorporation of 33P-dCTP (ICN #58201) using the procedure described at http://www.resgen.com/gf200pro.html, except that 33 mM of dATP, dTTP, and dGTP (Pharmacia #27-2035-01) and 1 U/μl of a ribonuclease inhibitor (Ambion #2690) were added.
Hybridization of the membrane with radioactive cDNA was performed by the procedures described at http://www.resgen.com/gf200pro.html, except that the last room temperature wash was performed in 2X SSC.
Nonparametric Probability Density Estimation by Optimization Theoretic Techniques, Technical report TR476-131-1, http://www.stat.rice.edu/stat/techr.html)
At the time of publication, this document, along with other publications pertaining to information security, was available on NIST's Computer Security Resource Clearinghouse internet page at http://csrc.nist.gov/publications.html.
The fasta_groups.txt file is particularly useful for subsequent Clustal X (Clustal X Help http://www-igbmc.u-strasbg.fr/BioInfo/ClustalX/Top.html; [ 4 5 ] ) and BLAST analyses (BLAST http://www.ncbi.nlm.nih.gov/BLAST/; [ 6 ] ). The second output file, group_seqs.txt, contains the group name and all sequences from the group.