Words similar to htk-

Example sentences for: htk-

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Cell lines were created by incorporation of retroviral vectors into the genome and expression of Tag from either the retroviral LTR (considered to be a strong promoter) or the herpes virus thymidine kinase promoter, internal to the viral LTR [ 1 ] . In our established NIH 3T3 cell line, Tag is expressed from the htk promoter at considerably lower levels than from the LTR for a considerable time after transduction [unpublished observations, and [ 11 ] ]. However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).
Polyclonal populations produced by transduction of htk-Tag vectors that were passaged without selection, displayed expression levels equivalent to or higher than LTR-Tag cell lines (Frisa and Jacobberger, unpublished data).