Words similar to htk
Example sentences for: htk
How can you use “htk” in a sentence? Here are some example sentences to help you improve your vocabulary:
Finally, the htk lines here varied significantly in terms of Tag expression potential, and in terms of the rate at which expression decreased as a function of cell density.
Originally, we pursued a strategy of reduced Tag expression from the htk promoter as a means of producing immortalized, differentiated cells that may have a reduced transformation phenotype.
The data presented here show that LTR driven Tag expressing cell lines either increase Tag during exponential growth despite a progressively increasing G 1 phase and contact inhibition (density) or the levels to not change significantly, whereas those created with the htk promoter show varying degrees of reduced expression, that is consistent in magnitude between experiments, as the cell cycle lengthens.
, [ 4 6 ] ]. In this study, the expression potential of cell lines was not related to proliferation related changes in expression of Tag, emphasizing that clonal variation for whatever reason (which includes promoter strength) accounts for the large range of htk related expression.
Cell lines were created by incorporation of retroviral vectors into the genome and expression of Tag from either the retroviral LTR (considered to be a strong promoter) or the herpes virus thymidine kinase promoter, internal to the viral LTR [ 1 ] . In our established NIH 3T3 cell line, Tag is expressed from the htk promoter at considerably lower levels than from the LTR for a considerable time after transduction [unpublished observations, and [ 11 ] ]. However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).
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