Example sentences for: htk

How can you use “htk” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Originally, we pursued a strategy of reduced Tag expression from the htk promoter as a means of producing immortalized, differentiated cells that may have a reduced transformation phenotype.

  • For example if an LTR Tag line and htk Tag line with equal expression potential were co-cultured, over time, one would expect the LTR driven line to dominate.

  • Cell lines were created by incorporation of retroviral vectors into the genome and expression of Tag from either the retroviral LTR (considered to be a strong promoter) or the herpes virus thymidine kinase promoter, internal to the viral LTR [ 1 ] . In our established NIH 3T3 cell line, Tag is expressed from the htk promoter at considerably lower levels than from the LTR for a considerable time after transduction [unpublished observations, and [ 11 ] ]. However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).

  • This supports the idea that the density independent expression in the htk lines is set by the clone specific effects (e.g.

  • We noticed however, that initially slow growing cell lines produced with htk driven gene expression needed to be cloned early to prevent overgrowth of more rapidly growing cells that expressed high levels of Tag.


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