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F98 rat glioma cells (ATCC, http://www.atcc.org/, Manassas, VA) were also used for comparative purposes in this study, and were cultured as described for SH-SY5Y cells.
Primers were designed with the Primer3 software http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiand were synthesized by Sigma-Genosys.
In fact, all 31 amino acids (34 missense/nonsense mutations total) for human myocilin documented in the Human Gene Mutation Database http://uwcmml1s.uwcm.ac.uk/uwcm/mg/search/5584221.htmlto cause glaucoma are conserved in rabbit myocilin (Fig 2).
A commentary on this article can be found at http://www.biomedcentral.com/1471-8219/2/5
The LocusLink (http://www.ncbi.nlm.nih.gov/LocusLink/) accession number for the KRAS2 sequence discussed in this paper is 3845; the GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) accession number for the KRAS2 sequence discussed in this paper is NT_009714.