Words similar to huape
Example sentences for: huape
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Some sequence entries represented the same protein, called by different names or isolated in different screens, including many entries for huAPE1, Drosophila Rrp 1 protein (~40% identical to the mammalian APE1 in the C-terminal third of the protein), Xth from E. coli , and counterparts of this and exodeoxyribonuclease (exo A) sequences from many bacteria, which are about 25% identical to mammalian APE1.
, rapidly releasing substrate/product), relatively non-specific DNase1 as opposed to the processive, highly specific huAPE1.
We then demonstrated that the shared molegos served a similar role in substrate binding by comparing the DNA binding profile of huAPE1 with that for the less specific enzyme DNase 1. The molegos present in both enzymes interact with target DNA in a similar fashion, while residues in molegos distinctive for APE1 control specificity by binding primarily to the bases around the apurinic site.
Mutations that greatly diminish the enzymatic activity of huAPE1 do not affect, and may even increase binding to damaged DNA, while non-specific DNA binding remains low [ 16 18 ] . Further, mutations that have little effect on APE activity in vitro prevent complementation of DNA repair deficient E. coli . As seen with other DNA repair enzymes [ 19 ] , specificity determining residues, as yet unidentified for APEs, must be distinct from those involved in phosphorolysis.
The 12 motifs are also structurally conserved, as demonstrated by the low RMSD values between segments in the crystal structures of bacterial ExoIII and of huAPE1.
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