Words similar to hrp-streptavidin
Example sentences for: hrp-streptavidin
How can you use “hrp-streptavidin” in a sentence? Here are some example sentences to help you improve your vocabulary:
1. The binding of these biotinylated proteins to the wells, after washing with TS, was determined by adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatin and 0.05 % Tween 20 for 30 min at 37°C.
To detect binding of CD4 to CAP treated and control gp120, wells coated with graded amounts of gp120 and postcoated as described above were reacted with biotinylated sCD4 (1 μg/well) in PBS containing 100 μg/ml BSA for 18 h at 4°C, washed with TS and the bound biotinylated CD4 was determined after adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatine and 0.05% Tween 20 for 30 min at 37°C.
After incubation for 1 h at 37°C the wells were washed and HRP-streptavidin (125 ng/ml in PBS containing 10% goat serum; 100 μl/well) was added.
After 4 h at 20°C, the wells were washed and bound gp120 was detected by addition of biotinyl-sCD4 (1 μg) followed by HRP-streptavidin as described above.
After incubation for 1 h at 37°C, the wells were washed as described above and HRP-streptavidin (125 ng/ml in PBS containing 10% goat serum; 100 μl/well) was added.