Words similar to hrp-streptavidin
Example sentences for: hrp-streptavidin
How can you use “hrp-streptavidin” in a sentence? Here are some example sentences to help you improve your vocabulary:
After incubation for 1 h at 37°C, the wells were washed as described above and HRP-streptavidin (125 ng/ml in PBS containing 10% goat serum; 100 μl/well) was added.
To detect binding of CD4 to CAP treated and control gp120, wells coated with graded amounts of gp120 and postcoated as described above were reacted with biotinylated sCD4 (1 μg/well) in PBS containing 100 μg/ml BSA for 18 h at 4°C, washed with TS and the bound biotinylated CD4 was determined after adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatine and 0.05% Tween 20 for 30 min at 37°C.
After 4 h at 20°C, the wells were washed and bound gp120 was detected by addition of biotinyl-sCD4 (1 μg) followed by HRP-streptavidin as described above.
1. The binding of these biotinylated proteins to the wells, after washing with TS, was determined by adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatin and 0.05 % Tween 20 for 30 min at 37°C.
After incubation for 1 h at 37°C the wells were washed and HRP-streptavidin (125 ng/ml in PBS containing 10% goat serum; 100 μl/well) was added.