Words similar to hrp-conjugated
Example sentences for: hrp-conjugated
How can you use “hrp-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
One quarter of each fraction was subjected to SDS-PAGE and immunoblot analysis using the 9E10 monoclonal antibody against the myc epitope (Oncogene Research Products, Cambridge, MA), followed by HRP-conjugated goat anti-mouse IgG and ECL detection reagent (Amersham Corp, Arlington Hts.
The upper half was probed with anti-HA antibody (4 μg/ml), followed by HRP-conjugated anti-mouse IgG (1:5,000, CalBiochem), and the signal was detected using ECL reagents (Amersham Pharmacia Biotech UK Ltd.).
Cells were fixed with 4% paraformaldehyde, blocked with BSA, immunostained using anti-phosphorylated ERK1/2, and visualized using HRP-conjugated secondary antibody and bisbenzamidine substrate.
Guinea pig IgG was detected with HRP-conjugated anti-IgG.
After washing in Tris-buffered saline with tween 20 (TBST), blots were incubated for one hour at room temperature with HRP-conjugated secondary antibody, washed again and detected by chemiluminescence.