Words similar to hrp-catalyzed

Example sentences for: hrp-catalyzed

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Although the location of activated NADPH oxidase complex in phagolysosomes has been reported, the release of either H 2 O 2 or O 2 •- in situ upon stimulation with PMA has not been directly shown in live cells [ 10 11 ] . In order to directly measure the oxidative activity in lysosomes, I use a reduced fluorescein derivative that is conjugated with BSA, OxyBURST Green H 2 HFF BSA, as shown in Figure 1. Spectrofluorimetric analysis indicates that this reduced compound is non-fluorescent, and becomes fluorescent upon HRP-catalyzed oxidation.
The HRP-catalyzed oxidation of this compound was determined spectroscopically as follows.
To test this possibility, effect of HRP-catalyzed oxidation on pH-dependence of fluorescein-dextran was investigated in vitro . As shown in Figure 5, increase in oxidation showed no effect on the pH-dependence of fluorescein-dextran.