Words similar to hrp
Example sentences for: hrp
How can you use “hrp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Bound HRP was detected using the test kit from Kirkegaard & Perry Laboratories following the manufacturer's protocol, and the absorbance was read at 450 nm.
HRP labeled goat anti-rabbit IgG was from Southern Biotechnology Associates, Inc., Birmingham, AL.
Detection antibodies (all from Dako; 1:5,000 for 1 h at room temperature) were mouse-anti-goat Ig, mouse-anti-rat Ig, rabbit-anti-mouse Ig, and porcine-anti-rabbit Ig-HRP [ 66 ] . To visualize all transferred proteins, we used the ECL protein biotinylation labeling modules (RPN 2202, Amersham) and streptavidin alkaline phosphatase (V020402, Amersham) in accordance with the manufacturer's instructions.
Ears were subsequently reacted for acetylated tubulin to reveal the pattern of innervation in addition to the DiI tracing with a different technique as previously described [ 14 ] . Briefly, dissected ears were incubated with 1:500 anti-acetylated tubulin antibodies (Sigma, St. Louis) followed by secondary antibodies conjugated to HRP.
The following day, sections were washed in PB and incubated in avidin-biotin-HRP complex (1:200, Vector Labs, Burlingane, CA) for at least 3 hours at room temperature.
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