Words similar to hrp
Example sentences for: hrp
How can you use “hrp” in a sentence? Here are some example sentences to help you improve your vocabulary:
After 4 h at 20°C the wells were washed and bound gp120 was detected by addition of biotinyl sCD4 (1 μg) followed by HRP streptavidin.
These solutions were then incubated at 37°C for 30 min, in the absence or presence of H 2 O 2 and HRP (final concentrations are 1 μM and 4 μg/mL, respectively).
Fibrosarcoma PAI-1 and wtPAI-1 were transferred onto nitrocellulose paper and the proteins were revealed with anti-human PAI-1 monoclonal antibodies, followed by horseradish peroxidase (HRP) conjugated rabbit anti-mouse antibody, and enhanced chemiluminescence (ECL).
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
After 2 h at 37°C bound HRP was detected using the kit from Kirkegaard & Perry Laboratories.
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