Words similar to hnt

Example sentences for: hnt

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To test whether such conditions induce dinucleoside polyphosphates in hnt2- or Hnt2+ cells, we incubated ADE2 hnt2 and ADE2 HNT2 cells in water, 1 M sorbitol, 2 mM CdCl 2 , 46 °C heat shock, 10 mM caffeine, or in rich media for two hours and determined dinucleoside polyphosphate levels.
While Dcps enzymes are specific for hydrolysis of cap structures such as 7meGpppG [ 28, 29], prototypical Hint enzymes such as rabbit Hint and yeast Hnt1 hydrolyze adenosine 5'-monophosphoramide substrates such as AMP-lysine to AMP plus lysine [ 30].
Other than effects on the concentrations of intracellular nucleotides, neither deletion of HNT2 nor mutation of His109 of HNT2 had phenotypic consequences.
Increases in dinucleoside polyphosphates in Hnt2+ cultures cannot be attributed to thermal inactivation of Hnt2 because the presence of HNT2 plasmids continues to reduce incremental increases in dinucleoside polyphosphates even in the fourth hour of the heat shock.
Our analysis shows that the corresponding residue, His109 of Hnt2, is required for hydrolysis of ApppN and AppppN substrates in vivo .