Words similar to hnf
Example sentences for: hnf
How can you use “hnf” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pgk promoter was deleted from 25% of transfected Hnf3αloxPNeo ES cells, using the approach described in materials and methods.
This in turn implies that, following electroporation of this targeting vector into Hnf3αΔpgk-Neo ES cells, 100% of G418 resistant colonies would have undergone homologous recombination.
In contrast, we predicted that homologous recombination between the truncated Neo sequences in the targeting vector and Neo sequences within Hnf3αΔpgk-Neo ES cells would reconstitute expression of active neomycin phosphotransferase and so confer resistance to G418.
In addition, mice lacking a single Hnf3 α allele have no observable phenotype, so that introduction of transgenes into this locus will not inherently affect embryonic development [ 14, 15].
This plasmid was introduced into Hnf3αΔpgk-Neo ES cells by electroporation and fifteen colonies were collected that were resistant to G418.