Words similar to hepes-buffered
Example sentences for: hepes-buffered
How can you use “hepes-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:
In a subset of experiments, GC were cultured in a defined serum-free medium of HEPES-buffered DMEM:F10 (1:1) containing insulin (300 mU/ml), hydrocortisone (40 ng/ml), transferrin (5 μg/ml), bovine serum albumin (4 mg/ml, Bovine Albumin Fraction V, albumin = 95%),
Oocytes were transferred into tubes containing Hepes-buffered TCM-199 (Gibco BRL, Grand Island, NY) supplemented with 0.57 mM cysteine, 10 ng/ml epidermal growth factor (Sigma), 100 IU/ml penicillin, 100 μg/ml streptomycin, 0.25 ng/ml amphotericin, 0.01 IU/ml porcine FSH (Sioux Biochemicals, Sioux Center, IA), 0.01 IU/ml porcine LH (Sioux Biochemicals) and 0.1% (w:v) polyvinyl alcohol (maturation medium) [ 50], and shipped to the laboratory overnight (20-24 h) at 38.
Labeled cells were incubated in 1 ml HEPES-buffered saline solution (HBS, composition in mM: NaCl, 145; KCl, 5; MgSO 4 , 1; CaCl 2 , 1.8; glucose, 10; HEPES, 10; pH 7.4) at 37°C for 30 min twice to wash out the unincorporated label.
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.
Unless otherwise indicated, HEPES-buffered saline (HBS) contained 140 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 10 mM D-glucose, 1.8 mM CaCl 2 , 15 mM HEPES, 0.1% bovine serum albumin, pH adjusted to 7.40 at 37°C with NaOH.
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