Words similar to hepes-buffered

Example sentences for: hepes-buffered

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Aspirated oocytes that had an evenly granulated cytoplasm and were surrounded by at least two uniform layers of compact cumulus cells were selected and washed three times in Hepes-buffered synthetic oviductal fluid medium [ 58].
Bt, Bacillus thuringiensis ; APN, aminopeptidase N; GPI, glycosyl-phosphatidylinositol; BBMV, brush border membrane vesicles; LpNA, leucine-p-nitroanilide; PIPLC, phosphatidylinositol-specific phospholipase C; HBS, Hepes-buffered saline; SPR, surface plasmon resonance
Labeled cells were incubated in 1 ml HEPES-buffered saline solution (HBS, composition in mM: NaCl, 145; KCl, 5; MgSO 4 , 1; CaCl 2 , 1.8; glucose, 10; HEPES, 10; pH 7.4) at 37°C for 30 min twice to wash out the unincorporated label.
Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
Unless otherwise indicated, HEPES-buffered saline (HBS) contained 140 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 10 mM D-glucose, 1.8 mM CaCl 2 , 15 mM HEPES, 0.1% bovine serum albumin, pH adjusted to 7.40 at 37°C with NaOH.