Words similar to hepes-buffered
Example sentences for: hepes-buffered
How can you use “hepes-buffered” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cumulus cells were removed by vortexing with 0.1% (w:v) hyaluronidase and denuded oocytes were transferred into 100 μl of Hepes-buffered TCM-199 supplemented with 7.5 μg/ml cytochalasin B (Sigma) and 10% FCS with the osmolarity adjusted to 300 mOsm by adding sorbitol (manipulation medium) at 29 or 42 h after IVM.
In a subset of experiments, GC were cultured in a defined serum-free medium of HEPES-buffered DMEM:F10 (1:1) containing insulin (300 mU/ml), hydrocortisone (40 ng/ml), transferrin (5 μg/ml), bovine serum albumin (4 mg/ml, Bovine Albumin Fraction V, albumin = 95%),
Culture conditions for GC were similar to those described previously by our laboratory and others [ 4 18 22 23 ] . The basic culture medium consisted of HEPES-buffered DMEM: F10 (1:1) supplemented with fetal bovine serum (FBS, 10%), penicillin (50 U/ml), streptomycin (50 μg/ml), gentamicin (57 ng/ml) and amphotericin (2.
Aspirated oocytes that had an evenly granulated cytoplasm and were surrounded by at least two uniform layers of compact cumulus cells were selected and washed three times in Hepes-buffered synthetic oviductal fluid medium [ 58].
Oocytes were transferred into tubes containing Hepes-buffered TCM-199 (Gibco BRL, Grand Island, NY) supplemented with 0.57 mM cysteine, 10 ng/ml epidermal growth factor (Sigma), 100 IU/ml penicillin, 100 μg/ml streptomycin, 0.25 ng/ml amphotericin, 0.01 IU/ml porcine FSH (Sioux Biochemicals, Sioux Center, IA), 0.01 IU/ml porcine LH (Sioux Biochemicals) and 0.1% (w:v) polyvinyl alcohol (maturation medium) [ 50], and shipped to the laboratory overnight (20-24 h) at 38.
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