Example sentences for: hepes

How can you use “hepes” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Control recording saline for two-electrode voltage clamp contained (in mM): 100 NaCl, 5 MgCl 2 , and 5 HEPES, pH 7.3.

  • 15 (50 mM HEPES/KOH, pH 7.5, 150 mM KCl, 0.02% Nonidet P-40, 5 mM magnesium acetate, 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).

  • RPMI 1640 culture medium was supplemented with 5% heat inactivated FCS, 2 mM glutamine, 5 mM HEPES, 5 × 10 -5M 2-ME, 100 U/ml penicillin, 20 μg/ml gentamycin (pH 7.2-7.

  • The neurons (chamber slides) were rinsed twice with fresh Locke's buffer containing physiological levels of calcium (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 2.3 mM; glucose, 5.6 mM; HEPES, 5 mM; pH 7.4) and immersed in the same solution (240 μl per chamber).

  • A cocktail was added that contained 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1mM DTT), 500 nM bacterially expressed 6 × His-UBCs, 100 nM 6 × His-tagged human E1, 0.5 μM ubiquitin aldehyde in a volume of 15 ul.


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