Words similar to hepes

Example sentences for: hepes

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To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.
After 5 min, cells were centrifuged (16,500 × g, 5 min), the pellets resuspended in buffer (50 mM HEPES pH 7.4, 50 mM KCl, 300 mM NaCl, 0.1 mM EDTA, 10% glycerol) and then placed on ice for an additional 20 min.
For cell-attached and inside-out single channel recordings, the pipette solution contained 135 mM KCl, 1.2 mM MgCl 2 , 5 mM glucose, 0.5 mM EGTA and 10 mM HEPES, pH 7.3.
Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.
Extracellular solution: NaCl 118, KCl 2.4, CaCl 2 1.8, MgCl 2 1.8, sodium pyruvate 5, glucose 5, HEPES 10, TTX 0.0003, pH 7.4 (with NaOH).