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Example sentences for: hepes

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After four hours, bacterial pellets were obtained, resuspended in 20 mM HEPES pH 7.9/ 80 mM KCl/6 mM MgCl 2 /1 mM Dithiothreitol/1 mM ATP/0.
In all recordings, the external (bath) solution contained (in mM): 145 tetraethylammonium (TEA) methanesulfonate (MS), 10 4-(2-Hydroxy ethyl)-1-piperazineethanesulfonic acid (HEPES), 15 glucose, 10 CaCl 2 , and 300 nM tetrodotoxin, pH 7.4, osmolality ~320 mOsm kg -1.
Adherent vascular lung neutrophils were isolated from rats as previously described [ 10 ] . Briefly, the lung was perfused with 50 ml of warm (37°C) Ca +2/Mg +2-free Hank's balanced salt solution (HBSS, pH 7.4) containing 2.5 mM HEPES, 0.5 mM EGTA and 4.4 mM NaHCO 3 at a rate of 22 ml/min and then lavaged 5-6 times with HBSS to remove alveolar macrophages and loosely adhered neutrophils.
Precipitates were washed four times in 20 mM Tris/HCI, pH 7.4; 150 mM NaCl; 0.5% Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.
Detached cells were washed twice with staining buffer (PBS containing 10% FCS, 0.1% sodium azide and 25 mM HEPES, pH 7.4) and stained as described previously [ 49 ] . Briefly, cells were transferred into the wells of round-bottom 96-well plates, the plates were centrifuged at 200 g for 30 seconds, the supernatant removed by shaking, and the cell pellets resuspended in 50 μl of staining buffer containing saturating concentration of anti-MHCII-FITC.