Words similar to hepes

Example sentences for: hepes

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RPMI 1640 culture medium was supplemented with 5% heat inactivated FCS, 2 mM glutamine, 5 mM HEPES, 5 × 10 -5M 2-ME, 100 U/ml penicillin, 20 μg/ml gentamycin (pH 7.2-7.
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
To prepare cell envelopes, cells were suspended in 0.01 M HEPES, pH 7.4 and sonicated on ice by using a Branson Sonifier (small tip, setting 7) [ 44 ] . The suspension was centrifuged at 12,000 x g for 2 minutes at 4°C to remove unbroken cells and debris.
Cells were propagated in T75 flasks in RPMI-1640 medium supplemented with 25 mM HEPES, 10% fetal calf serum, 200 mM L-glutamine and 40 μg/ml Tobramycin.
For adenoviral transduction, lymph nodes and spleen cells were isolated, treated with ACK lysing buffer for 3 min and washed in DMEM/10 mM HEPES (pH 7.2).