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Example sentences for: hepes

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These PG-1 solutions were also prepared in 100 mM NaCl containing 10 mM HEPES buffer (pH 7.4).
Cells were scraped into ice-cold lysis buffer: 50 mM HEPES, pH 7.4, 150 mM sodium chloride, 1.5 mM magnesium chloride, 10% glycerol, 1% Triton X-100, 1 mM EGTA, 200 μM sodium vanadate, 10 mM tetrasodium pyrophosphate, 100 mM sodium fluoride, 10 μg/ml leupeptin, and 10 μg/ml aprotinin.
Cells were diluted in buffer S and pelleted by centrifugation and resuspended in buffer F (18 % Ficoll 400, w/v; 20 mM HEPES (pH = 7.2), 0.5 mM MgCl 2 and protease inhibitors.
The adherent cells in the well were washed three times with 1.0 ml of PBS, and the cells overlaid with 400 μl of lysis buffer (1% Triton X-100 in pH 7.5, 20 mM HEPES buffer with 0.1 mM EDTA), scraped, and collected into a glass vial for tocopherol analysis by HPLC, and for protein determination by the BCA protein assay (Pierce, Rockford, IL).
After 1 h in an incubator, the cells were rinsed with fresh Mg 2+-free Locke's solution (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 2.3 mM; glucose, 5.6 mM; HEPES, 5 mM; pH 7.4), and returned to the incubator for an additional 15 minutes prior to initiating the imaging experiments.