Example sentences for: hepes

How can you use “hepes” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Unless otherwise indicated, HEPES-buffered saline (HBS) contained 140 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 10 mM D-glucose, 1.8 mM CaCl 2 , 15 mM HEPES, 0.1% bovine serum albumin, pH adjusted to 7.40 at 37°C with NaOH.

  • To immobilize the LPS, 60 μl of a 1 mg/ml LPS preparation was dispersed in 100 mM NaCl containing 10 mM HEPES buffer (pH 7.4), and passed across the chip's surface for 30 min, at flow rate of 2 μl/min.

  • The pellet was resuspended in 50 μl B/EDTA/sucrose (10 mM HEPES pH 7.8, 15 mM KCl, 5 mM EDTA, 5% sucrose) and stored at -75°C.

  • For each reaction, 5 × 10 3cpm of labeled probe was incubated with 5 μg of crude nuclear proteins from Sertoli cells and 1.0 μg of poly dI:dC in a buffer containing 5 mM Hepes pH 7.9, 26 % glycerol, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 150 mM KCl and 5 mM MgCl at room temperature for 15 min.

  • Mitochondria were isolated from about 3 × 10 8cells according to published procedures [ 26 27 ] . The final mitochondrial pellet was suspended in suspension buffer (140 mM KCl, 0.3 mM EDTA, 5 mM MgCl 2 , 10 mM HEPES, pH 7.4) to give a protein concentration of 3-4 mg/ml.


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