Words similar to hepes

Example sentences for: hepes

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Surprisingly, the ester moiety of acetylcholine does not seem to interact with the cys-loop of the receptor, as previously suggested by photoaffinity labeling [ 12 ] , but is engaged in similar type of interaction as that observed with the acidic group of HEPES in the AChBP crystal structure [ 6 ] . It is possible that the reactive di-azo group of the photosensitive agonist used in photoaffinity labeling would extend towards the cys-loop, while the conformation of the bound agonist remains in agreement with our model.
Mitochondria were isolated from about 3 × 10 8cells according to published procedures [ 26 27 ] . The final mitochondrial pellet was suspended in suspension buffer (140 mM KCl, 0.3 mM EDTA, 5 mM MgCl 2 , 10 mM HEPES, pH 7.4) to give a protein concentration of 3-4 mg/ml.
0 MΩ were filled with filtered intracellular solution containing 145 mM KCl, 2 mM MgCl 2 , 1 mM EGTA, 25 mM HEPES (pH 7.4).
They were incubated with 20,000 units/ml β-glucuronidase from bovine liver (Sigma) in 50 mM Hepes (pH 7.5) containing 150 mM NaCl, 5 mM β-glycerophosphate, 0.5% human serum albumin, 0.5% saponin with or without 10 mM M6P overnight on ice.
Precipitates were washed four times in 20 mM Tris/HCI, pH 7.4; 150 mM NaCl; 0.5% Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF, and equilibrated in 20 mM HEPES, pH 7.4, 100 mM potassium acetate, 1 mM DTT.