Words similar to hepes

Example sentences for: hepes

How can you use “hepes” in a sentence? Here are some example sentences to help you improve your vocabulary:

Cultures were lysed in 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 1 mM DTT, 1% Nonidet P-40, 10% glycerol, 1 mM NaF, 0.1 mM sodium orthovanadate, and protease inhibitors for 30 minutes at 4°C.
Monolayers were then washed with cold PBS, pH 7.5, and solubilized in 1.0 ml of cell extraction buffer (50 mM HEPES, pH 7.5, 1% Triton X-100, 0.1% SDS, 0.25% deoxycholate, 150 mM sodium chloride, 1 mM EDTA, and protease inhibitors.
The fractions containing the major peak were collected, and the solvent was changed to 20 mM Hepes (pH8.
To prepare cell envelopes, cells were suspended in 0.01 M HEPES, pH 7.4 and sonicated on ice by using a Branson Sonifier (small tip, setting 7) [ 44 ] . The suspension was centrifuged at 12,000 x g for 2 minutes at 4°C to remove unbroken cells and debris.
Complexes were recovered with Protein A Sepharose, washed twice with ice cold NP40 wash buffer, washed twice with ice cold Rlk kinase buffer (30 mM HEPES pH 7.4, 150 mM NaCl, 5 mM MgCl