Words similar to hepes

Example sentences for: hepes

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After 1 h in an incubator, the cells were rinsed with fresh Mg 2+-free Locke's solution (NaCl, 154 mM; KCl, 5.6 mM; NaHCO 3 , 3.6 mM; CaCl 2 , 2.3 mM; glucose, 5.6 mM; HEPES, 5 mM; pH 7.4), and returned to the incubator for an additional 15 minutes prior to initiating the imaging experiments.
Nuclei were prepared from HeLa cells by standard procedures [ 35] and resuspended in 10 packed nuclei volumes of buffer A (10 mM Hepes (pH 7.9) 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 0.5 mM PMSF).
Incorporation of tritiated lysine into tRNA was measured by modification of the protocol of Hou [ 51 ] . Reactions contained 10 micrograms of total protein in 20 mM KCl, 10 mM MgCl 2 , 4 mM dithiothreitol, 2 mM ATP, 50 mM Na HEPES pH 7.5, 20 μM lysine (10 μCi 3H lysine), and were performed with or without 15 μg yeast tRNA (Sigma) in a total volume of 60 μl.
Patch pipettes were filled with a solution containing (in mM): 120 CsF, 33 KOH, 2 MgCl 2 , 1 CaCl 2 , 0.1 spermine, 10 HEPES, and 11 EGTA (pH 7.4).
Monolayers were then washed with cold PBS, pH 7.5, and solubilized in 1.0 ml of cell extraction buffer (50 mM HEPES, pH 7.5, 1% Triton X-100, 0.1% SDS, 0.25% deoxycholate, 150 mM sodium chloride, 1 mM EDTA, and protease inhibitors.