Words similar to hbs

Example sentences for: hbs

How can you use “hbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

To assemble Cdc14 beads with Net1N for two assays (Figure 4B, detection method: Western blotting), 2 μl of rabbit-anti-GST antibodies were absorbed to 40 μl of protein A beads (Sigma) in 100 μl of HBS [20 mM HEPES (pH 7.2), 150 mM NaCl] + 2 mM EDTA + 0.1 mg/ml BSA + 1 mM DTT (HEBD) buffer for 1 hr.
Vital dye uptake was determined as previously described [ 1 2 3 ] . Briefly, an aliquot (2 ml) of dispersed cells suspended in HBS at 37°C was placed in a cuvette.
Ca 2+-free HBS contained 0.3 mM EGTA and the same salts as HBS without added CaCl 2 . Fura-2 acetoxymethyl ester (fura-2/AM), ethidium bromide, YO-PRO-1 and POPO-3 were obtained from Molecular Probes (Eugene, OR, USA).
Unless otherwise indicated, HEPES-buffered saline (HBS) contained 140 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 10 mM D-glucose, 1.8 mM CaCl 2 , 15 mM HEPES, 0.1% bovine serum albumin, pH adjusted to 7.40 at 37°C with NaOH.
Fura-2-loaded BAECs were suspended in HBS, placed in a cuvette at 37°C, and the response to extracellular application of MTX was recorded as a function of time (Fig.