Example sentences for: hbss

How can you use “hbss” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After incubation the tubes were centrifuged at 300 g and cell pellet was washed gently in Hank's balanced salt solution (HBSS) at least thrice.

  • Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).

  • Cells were continuously superperfused with HBSS containing the indicated drugs.

  • Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Dulbecco's phosphate buffered saline (DPBS), antibiotic/antimycotic, 1% trypsin/EDTA, Hanks Balanced Salt Solution (HBSS) and TRIZOL were purchased from Invitrogen Gibco Cell Culture (Carlsbad, CA).

  • Choroid plexus cells were isolated as described [ 46 ] . Briefly, choroid plexus from adult male Sprague-Dawley rats (175-200 g) were dissected into cold Locke's buffer, rinsed and enzymatically dissociated for 10 minutes at 37° C in Hank's Balanced Salt Solution (HBSS) containing 330 ug/ml Pronase I and 260 ug/ml DNase I. The cells were pelleted and choroid plexus cells were released after an additional digest with 130 ug/ ml DNase I in HBSS.


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