Words similar to hbss
Example sentences for: hbss
How can you use “hbss” in a sentence? Here are some example sentences to help you improve your vocabulary:
After incubation the tubes were centrifuged at 300 g and cell pellet was washed gently in Hank's balanced salt solution (HBSS) at least thrice.
Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).
Cells were continuously superperfused with HBSS containing the indicated drugs.
Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Dulbecco's phosphate buffered saline (DPBS), antibiotic/antimycotic, 1% trypsin/EDTA, Hanks Balanced Salt Solution (HBSS) and TRIZOL were purchased from Invitrogen Gibco Cell Culture (Carlsbad, CA).
Choroid plexus cells were isolated as described [ 46 ] . Briefly, choroid plexus from adult male Sprague-Dawley rats (175-200 g) were dissected into cold Locke's buffer, rinsed and enzymatically dissociated for 10 minutes at 37° C in Hank's Balanced Salt Solution (HBSS) containing 330 ug/ml Pronase I and 260 ug/ml DNase I. The cells were pelleted and choroid plexus cells were released after an additional digest with 130 ug/ ml DNase I in HBSS.
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