Words similar to hbss
Example sentences for: hbss
How can you use “hbss” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, TK-1 cells were suspended in Hank's Balanced salt solution (HBSS) and radiolabeled by incubating TK-1 cells at 2 × 10 7cells/ml with 30 μCi Na 51CrO 4 /ml (New England Nuclear, Natick, MA) at 37°C for 60 min.
RSV stocks were resuspended in HBSS containing 0.5% bovine serum albumin and 100 mM magnesium sulfate and frozen at -70° C.
To make lysates, transfected cells were washed with cold HBSS, scraped, lysed on ice in 300 μl lysis buffer (20 mM Tris, PH 7.4, 150 mM NaCl, and 0.5% Triton X-100, 1mM EDTA) containing protease inhibitors.
Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).
O 2 - : O 2 - generation was determined by the SOD-inhibitable reduction of FCC [ 7 ] . Briefly, O 2 - release from microglia was measured in the presence of FCC (50 μM) and HBSS, with or without SOD (700 Units).