Words similar to hbss
Example sentences for: hbss
How can you use “hbss” in a sentence? Here are some example sentences to help you improve your vocabulary:
The suspension obtained by shaking the bottle was filtered through 0.5 mm metal mesh and washed with HBSS including 2% bovine serum albumin (BSA).
Chemotactic responsiveness of lung neutrophils towards bacterially-derived n-formyl-methionyl-leucyl-phenylalanine (fMLP) was quantified using a Neuroprobe 48-microwell chemotaxis chamber and 5-μm pore size Nucleopore filters [ 12 13 ] . Cells (2 × 10 6cells/ml), suspended in HBSS containing 0.5% BSA, were incubated in the upper wells of the chamber at 37°C with fMLP (50 nM) or medium in the lower wells.
Choroid plexus cells were isolated as described [ 46 ] . Briefly, choroid plexus from adult male Sprague-Dawley rats (175-200 g) were dissected into cold Locke's buffer, rinsed and enzymatically dissociated for 10 minutes at 37° C in Hank's Balanced Salt Solution (HBSS) containing 330 ug/ml Pronase I and 260 ug/ml DNase I. The cells were pelleted and choroid plexus cells were released after an additional digest with 130 ug/ ml DNase I in HBSS.
Confluent HUVEC were washed with HBSS and incubated for 24 hrs at 37°C with various agonists in the medium: MEDIUM only; DMSO 0.5 % (vehicle for camptothecin); tumor necrosis factor-α (TNF) from Alexis Biochemicals (San Diego, CA) 100 or 1000 U/mL; cycloheximide (CHX) 50 μg/mL; TNF 1000 U/mL with cycloheximide 50 μg/mL (TNF/CHX); lipopolysaccharide (LPS) E. coli 055:B5 from Calbiochem (San Diego, CA) 10 μg/mL; camptothecin (CPT) from Sigma (St.
After 7 days in culture, cells were loaded with 1.5 μM fura-2 AM (Molecular Probes, Eugene, OR) in HBSS and 1 mg/ml bovine serum albumin (BSA) for 30 minutes at 22°C and washed in HBSS for 30 minutes at 22°C.
Loading...