Example sentences for: hbss

How can you use “hbss” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were continuously superperfused with HBSS containing the indicated drugs.

  • After 7 days in culture, cells were loaded with 1.5 μM fura-2 AM (Molecular Probes, Eugene, OR) in HBSS and 1 mg/ml bovine serum albumin (BSA) for 30 minutes at 22°C and washed in HBSS for 30 minutes at 22°C.

  • Neutrophils were recovered, after digestion of the lung tissue for 25 min with 60 U/ml collagenase D (specific activity 0.22 Wunsch unit = 800 IU, low ancillary protease activity, Boehringer Mannheim, Indianapolis, IN, USA), prepared in HBSS containing 10% fetal bovine serum (FBS) and 0.01% DNAse 1 (Sigma Chemical Co., St. Louis, MO, USA), followed by filtering (220 μm).

  • Confluent HUVEC were washed with HBSS and incubated for 24 hrs at 37°C with various agonists in the medium: MEDIUM only; DMSO 0.5 % (vehicle for camptothecin); tumor necrosis factor-α (TNF) from Alexis Biochemicals (San Diego, CA) 100 or 1000 U/mL; cycloheximide (CHX) 50 μg/mL; TNF 1000 U/mL with cycloheximide 50 μg/mL (TNF/CHX); lipopolysaccharide (LPS) E. coli 055:B5 from Calbiochem (San Diego, CA) 10 μg/mL; camptothecin (CPT) from Sigma (St.

  • At the end of the incubation period, the supernatant was removed and the monolayers were washed twice with HBSS.


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