Example sentences for: gibco

How can you use “gibco” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Oocytes were transferred into tubes containing Hepes-buffered TCM-199 (Gibco BRL, Grand Island, NY) supplemented with 0.57 mM cysteine, 10 ng/ml epidermal growth factor (Sigma), 100 IU/ml penicillin, 100 μg/ml streptomycin, 0.25 ng/ml amphotericin, 0.01 IU/ml porcine FSH (Sioux Biochemicals, Sioux Center, IA), 0.01 IU/ml porcine LH (Sioux Biochemicals) and 0.1% (w:v) polyvinyl alcohol (maturation medium) [ 50], and shipped to the laboratory overnight (20-24 h) at 38.

  • Cells were digested with mixture of 0.025% trypsin (Gibco BRL) and 0.01% EDTA (Sigma).

  • Biosource International, Rockville, MD, and GIBCO/Life Technologies, Grand Island, NY.

  • Bovine aortic endothelial cells were cultured as previously described [ 23] using Dulbecco's modified Eagles medium (GIBCO) supplemented with 10% fetal bovine serum (Hyclone, Logan UT), 100 μg/ml streptomycin and 100 μg/ml penicillin (complete-DMEM).

  • One microgram of RNA was reverse transcribed to complementary (c)DNA using 200U Moloney murine leukemia virua (M-MLV) reverse transcriptase (GIBCO BRL Life Technologies, Grand Island, NY, USA), 1U RNAse inhibitor (5 Prime 3 Prime, Boulder, CO, USA) and 20 pmol oligo dT primer in a total volume of 20 μl.


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