Words similar to gibco
Example sentences for: gibco
How can you use “gibco” in a sentence? Here are some example sentences to help you improve your vocabulary:
RNA was extracted using Trizol reagent (Gibco BRL) and the amount of RNA was quantitated by UV spectrophotometry.
G418-resistant colonies from BRCA1 antisense infected BG-1 cells and control vector LXSN-infected BG-1 cells were pooled, pretreated for 5 days in phenol red-free, DMEM/F-12 medium (Gibco/Life Technologies) supplemented with 10% charcoal/dextran treated serum (Hyclone, Logan, UT, USA), then plated at 2.5 × 10 6cells per 100mm dish in triplicate for growth curve analysis in the absence or presence of estrogen (10 -8mol/l; 17β-Estradiol; 1,3,5 (10) - Estratriene 3, 17β-diol; Sigma, St Louis, MO, USA).
After all the components were assembled, 200 units of MMLV reverse transcriptase (Gibco BRL Life Technologies, Bethesda, MA) were added.
05% collagenase and 0.25% trypsin (GIBCO, Grand Island, NY) in Ca 2+/Mg 2+-free HBSS.
RT-PCR was performed as described [ 62 ] . 20 μg RNA was treated with 1 unit of RNAse free DNase (Gibco BRL) (20 mM Tris-HCl pH 8.4, 50 mM KCL 2.5 mM MgCl2) for 15 minutes at room temperature.
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