Example sentences for: gibco-

How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All tissue culture supplies and the TRIzol reagent were purchased from Gibco-BRL.

  • All ES cell lines were cultured on mitotically inactivated primary embryonic fibroblasts in ES cell medium supplemented with recombinant leukemia inhibitory factor (LIF) as described elsewhere [ 22 ] . F3 cells were generated by introducing 100 μg of Not1 digested pΔHPRT plasmid into 1.5 × 10 8R1 ES cells by electroporation at 250 volts/500μf/resistance 8 using a BTX ECM600 electroporation system [ 7 ] . Cells containing neo were selected by supplementing the ES cell medium with 250 μg/ml Geneticin (G418, Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).

  • Transcript size was determined by comparison with 1 kb RNA ladder (Gibco-BRL) according to the manufacturer's instructions.

  • Total RNA was extracted from homogenized DRG samples using acid phenol extraction (TRIzol reagent, Gibco-BRL).

  • with 20 μg anti-DNP-IgE (IgE hybridoma = H1 DNP-ε-26) [ 16 ] dissolved in 200 μl HMEM (Gibco-BRL, Gaithersburg, MD) with PIPES buffer (0.


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