Example sentences for: gibco-

How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5 μl of 0.25% trypsin-EDTA (Gibco-BRL) was used as a positive control for the casein zymogram.

  • All tissue culture supplies and the TRIzol reagent were purchased from Gibco-BRL.

  • The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).

  • with 20 μg anti-DNP-IgE (IgE hybridoma = H1 DNP-ε-26) [ 16 ] dissolved in 200 μl HMEM (Gibco-BRL, Gaithersburg, MD) with PIPES buffer (0.

  • The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.


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