Words similar to gibco-
Example sentences for: gibco-
How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
First strand cDNA synthesis was performed at 42°C for 30 min in a 20 μl reaction containing: 5 μg RNA, 500 nM NEO A primer (5'-ATTGTCTGTTGTGCCCAGTCATA), 20 mM Tris-HCl (pH 8.4), 50 mM KCl 2.5 mM MgCl 2 , 10 mM DTT, 400 μM each dNTP, and 8 units Super Script II reverse transcriptase (Gibco-BRL).
To hybridize the arrays, purified and labeled cDNA targets were dissolved in 100 μl total volume of ExpressHyb solution (Clontech, Palo Alto, CA) containing 8 μg of polydA 40-60 (Amersham Pharmacia), 2 μg of yeast tRNA (Gibco-BRL), 10 μg of human Cot I DNA (Gibco-BRL).
Cells were transfected using Lipofectin (Gibco-BRL) containing p50-Luc (1 μg), pCMV-Gal (1 μg) and pcDNAp53 (50 ng) in a final volume of 400 μl as described in the manufacturers protocol.
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