Words similar to gibco-

Example sentences for: gibco-

How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:

First strand cDNA synthesis was performed at 42°C for 30 min in a 20 μl reaction containing: 5 μg RNA, 500 nM NEO A primer (5'-ATTGTCTGTTGTGCCCAGTCATA), 20 mM Tris-HCl (pH 8.4), 50 mM KCl 2.5 mM MgCl 2 , 10 mM DTT, 400 μM each dNTP, and 8 units Super Script II reverse transcriptase (Gibco-BRL).
Cells were grown in Dulbecco's modified Eagle's medium (DME) containing 10% FBS (Gibco-BRL), antibiotic-antimycotic at 1/100 (Gibco-BRL; 10,000 units/ml penicillin G, 10,000 μg/ml streptomycin sulfate, 25 μg/ml amphotericin B).
Cells containing Neo were selected by supplementing the ES cell medium with 300 μg/ml Geneticin (G418,Gibco-BRL) and negative selection against Hsv-tk gene expression was achieved by including 2 μM gancyclovir (Roche).
Cells were transfected using Lipofectin (Gibco-BRL) containing p50-Luc (1 μg), pCMV-Gal (1 μg) and pcDNAp53 (50 ng) in a final volume of 400 μl as described in the manufacturers protocol.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).