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Example sentences for: gibco-

How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:

5 μg of plasmid DNA was used in each sequencing reaction [ 10 61 ] . Initial sequences were determined by using T3 and T7 primers, and extended by using custom 17-18 nt primers (Gibco-BRL).
PCR was performed using a standard protocol as follows with a final volume of 50 μl; 5 μl 10X PCR buffer, 5 μl 2 mM dNTP, 5 μl forward primer (concentration 20 ng/ul), 5 ul reverse primer (concentration 20 ng/ul), 1.5 μl 50 mM MgCl, 0.25 μl Taq polymerase (5 U/μl), and 1 μl sample purified genomic DNA (concentration approximately 30 ng/μl) (PCR reagents, Gibco-BRL, Bethesda, MD).
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).
with 20 μg anti-DNP-IgE (IgE hybridoma = H1 DNP-ε-26) [ 16 ] dissolved in 200 μl HMEM (Gibco-BRL, Gaithersburg, MD) with PIPES buffer (0.
Total RNA was extracted from homogenized DRG samples using acid phenol extraction (TRIzol reagent, Gibco-BRL).