Example sentences for: gibco-

How can you use “gibco-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were grown in Dulbecco's modified Eagle's medium (DME) containing 10% FBS (Gibco-BRL), antibiotic-antimycotic at 1/100 (Gibco-BRL; 10,000 units/ml penicillin G, 10,000 μg/ml streptomycin sulfate, 25 μg/ml amphotericin B).

  • U251 and LN229 human glioma cell lines were maintained in DMEM supplemented with 10% fetal calf serum (Gibco-BRL, Gaithersburg, MD).

  • 5 μg of plasmid DNA was used in each sequencing reaction [ 10 61 ] . Initial sequences were determined by using T3 and T7 primers, and extended by using custom 17-18 nt primers (Gibco-BRL).

  • Ham's F12 medium, Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, type II collagenase, dispase, and Geneticin™ were supplied by Gibco-BRL (Rockville, MD, USA).

  • Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).


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