Words similar to gibcobrl

Example sentences for: gibcobrl

How can you use “gibcobrl” in a sentence? Here are some example sentences to help you improve your vocabulary:

The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
Each round of PCR consisted of 31 cycles at 94°C for 20 seconds, 55°C for 40 seconds, and 72°C for 45 seconds; Platinum ®Taq DNA polymerase (GibcoBRL, Baltimore, MD) was used for DNA amplification.
Cells were maintained in Medium 199/MCDB 105 (1:1) (Sigma, St. Louis, MO) with 5% fetal bovine serum (GibcoBRL, Rockville, MD) and 10 ug/ml gentamicin (GibcoBRL) in 5% CO 2 /95% air at 37°C as described previously [ 31 40 ] .
Complete methods for the stable transfection of HEK-293 cells to express the wild type receptor can be found in a previous report [ 13 ] . Both cell lines were seeded in 6-well dishes at an initial cell density of 1 × 10 6cells/well and were maintained in Dulbecco's modified Eagle's medium (DMEM; GibcoBRL) supplemented with 2 mM L-glutamine, 5% fetal bovine serum (FBS) (Gemini Bio-Products, Calabasas, CA), and 100 μg/ml gentamycin sulfate (Gemini Bio-Products, Calabasas, CA).
Total RNA was extracted from 13 samples, using the Trizol method (GibcoBRL).