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Example sentences for: gibcobrl

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Each round of PCR consisted of 31 cycles at 94°C for 20 seconds, 55°C for 40 seconds, and 72°C for 45 seconds; Platinum ®Taq DNA polymerase (GibcoBRL, Baltimore, MD) was used for DNA amplification.
The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
The SuperScript™ Choice System was used for (ds)-cDNA synthesis (GibcoBRL Life Technologies) using the T7-(dT) 24 primer 5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(dT) 24 -3' and 3 μg of mRNA for each reaction.
The reaction cocktail contained 1 ng EST plasmid DNA, 1.7 mM MgCl 2 , 1x reaction buffer (50 mM Tris pH 8.5, 20 mM KCl), and 0.1 units of Taq polymerase (GibcoBRL, Gaithersburg, MD).
The cultures were maintained in Minimum Essential Medium (MEM, GibcoBRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO), 1.4 mM glutamine (GibcoBRL, Grand Island, NY) and 20 μM 2-mercaptoethanol (Sigma, St. Louis, MO).