Words similar to gibcobrl

Example sentences for: gibcobrl

How can you use “gibcobrl” in a sentence? Here are some example sentences to help you improve your vocabulary:

2 μm) conditioned medium that was prepared by culturing the primary cell strain for two days during the exponential growth phase in MEM-alpha (Sigma, St. Louis, MO) with 15% fetal calf serum (Gemini, Woodland, CA), penicillin (100 U/ml), streptomycin (100 μg/ml), and glutamine (290 ng/ml) (GibcoBRL Life Technologies).
05%) (GibcoBRL Life Technologies) and diluted to 100 cells/ml.
The reaction cocktail contained 1 ng EST plasmid DNA, 1.7 mM MgCl 2 , 1x reaction buffer (50 mM Tris pH 8.5, 20 mM KCl), and 0.1 units of Taq polymerase (GibcoBRL, Gaithersburg, MD).
Reverse transcription of 2 μg of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II RNase H-Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 500 ng of random hexamers.
Each round of PCR consisted of 31 cycles at 94°C for 20 seconds, 55°C for 40 seconds, and 72°C for 45 seconds; Platinum ®Taq DNA polymerase (GibcoBRL, Baltimore, MD) was used for DNA amplification.