Words similar to gibcobrl
Example sentences for: gibcobrl
How can you use “gibcobrl” in a sentence? Here are some example sentences to help you improve your vocabulary:
The inhibitors used and their stock solutions were as follows: quin2-AM (Sigma, 2 mM), herbimycin A (GibcoBRL, 0.175 mM), chelerythrine (Sigma, 0.4 mM), cytochalasin D (Sigma, 8 μM), nocodazole (Sigma, 0.66 mM).
Each round of PCR consisted of 31 cycles at 94°C for 20 seconds, 55°C for 40 seconds, and 72°C for 45 seconds; Platinum ®Taq DNA polymerase (GibcoBRL, Baltimore, MD) was used for DNA amplification.
The reaction cocktail contained ~2 μg poly(A) +RNA, 1x reaction buffer (50 mM Tris-HCl pH 8, 75 mM KCl, 3 mM MgCL 2 , 50 μM dNTP, 10 mM DTT), 1 μg oligo dT, 3 μg random hexamer, and 400 units Superscript II (GibcoBRL).
05%) (GibcoBRL Life Technologies) and diluted to 100 cells/ml.
Reverse transcription of 2 μg of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II RNase H-Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 500 ng of random hexamers.