Example sentences for: gibcobrl

How can you use “gibcobrl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cultures were maintained in Minimum Essential Medium (MEM, GibcoBRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (Sigma, St. Louis, MO), 1.4 mM glutamine (GibcoBRL, Grand Island, NY) and 20 μM 2-mercaptoethanol (Sigma, St. Louis, MO).

  • 2 μm) conditioned medium that was prepared by culturing the primary cell strain for two days during the exponential growth phase in MEM-alpha (Sigma, St. Louis, MO) with 15% fetal calf serum (Gemini, Woodland, CA), penicillin (100 U/ml), streptomycin (100 μg/ml), and glutamine (290 ng/ml) (GibcoBRL Life Technologies).

  • The inhibitors used and their stock solutions were as follows: quin2-AM (Sigma, 2 mM), herbimycin A (GibcoBRL, 0.175 mM), chelerythrine (Sigma, 0.4 mM), cytochalasin D (Sigma, 8 μM), nocodazole (Sigma, 0.66 mM).

  • Transfections were performed in 6-well plates or 6 cm culture dishes using Lipofectamine (GibcoBRL) or FuGene6 (Roche) according to the manufacturer's protocol.

  • Total RNA was extracted from 13 samples, using the Trizol method (GibcoBRL).


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