Example sentences for: gibco-brl

How can you use “gibco-brl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • LPS B (Escherichia coli 026:B6) was obtained from Difco Laboratories (Detroit, MI); crystalline lyophilized domoic acid was purchased from Diagnostics Chemicals Ltd. (Oxford, CT) and was dissolved in LPS-free water obtained from GIBCO-BRL (Grand Island, NY) to prepare a 12.

  • Murine fibroblast NIH 3T3 cells, amphotropic PA317 packaging cells, and Phoenix E ecotropic packaging cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (GIBCO-BRL, Grand island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL, Grand island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 200 mM L-glutamine.

  • Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).

  • Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.

  • The cells were then harvested, and total RNA was extracted with TRIzol Reagent (GIBCO-BRL) according to the methods of the manufacturer.


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