Words similar to gibco-brl
Example sentences for: gibco-brl
How can you use “gibco-brl” in a sentence? Here are some example sentences to help you improve your vocabulary:
CRE8 and 293 cell lines were grown in Dulbecco's modified eagle medium (GIBCO-BRL, Rockville, MD, USA) supplemented with 10% fetal bovine serum.
Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.
LPS B (Escherichia coli 026:B6) was obtained from Difco Laboratories (Detroit, MI); crystalline lyophilized domoic acid was purchased from Diagnostics Chemicals Ltd. (Oxford, CT) and was dissolved in LPS-free water obtained from GIBCO-BRL (Grand Island, NY) to prepare a 12.
The cells were then harvested, and total RNA was extracted with TRIzol Reagent (GIBCO-BRL) according to the methods of the manufacturer.
Sub-confluent (80-90%) LNCaP cells propagated in T25 flasks were washed with 5 ml of plain RPMI 1640 medium and trypsinized with 5 ml trypsin/EDTA solution (GIBCO-BRL, Gaithersburg, MD).
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