Words similar to gact

Example sentences for: gact

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The Q758C variant was frequently found adjacent to the wild-type T7 RNA polymerase promoter (GACT at positions -11 through -8) but was also found adjacent to a single mutant promoter (GAC G ), double mutant promoters (GA TA , G T C A ), a triple mutant promoter (G TTA ), and a promoter in which all the residues differed from wild-type ( TGTA ). Overall, the 'generalist' polymerase appeared to prefer mutant promoters that contained a T at position -9, and G or A at -8 positions.
Despite the fact that this variant was extremely novel, it was nonetheless completely functional, and yielded as much or more polymerase expression when paired with its promoter G GTA than did the wild type polymerase paired with the wild type promoter (GACT) (Figure 2b).
The Q758C mutant has previously been assayed for its activity at position -8 with different bases, and was shown to prefer G or A at -8 compared to the wild type promoter (-8T) [ 7 ] . This finding was further borne out in our experiments; the Q758C:GAC G polymerase:promoter combination yielded substantially more T7 RNA polymerase as judged by gel electrophoresis, than did Q758C:GACT (wt) (Figure 2a).