Words similar to gact

Example sentences for: gact

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The Q758C variant was frequently found adjacent to the wild-type T7 RNA polymerase promoter (GACT at positions -11 through -8) but was also found adjacent to a single mutant promoter (GAC G ), double mutant promoters (GA TA , G T C A ), a triple mutant promoter (G TTA ), and a promoter in which all the residues differed from wild-type ( TGTA ). Overall, the 'generalist' polymerase appeared to prefer mutant promoters that contained a T at position -9, and G or A at -8 positions.
The Q758C mutant has previously been assayed for its activity at position -8 with different bases, and was shown to prefer G or A at -8 compared to the wild type promoter (-8T) [ 7 ] . This finding was further borne out in our experiments; the Q758C:GAC G polymerase:promoter combination yielded substantially more T7 RNA polymerase as judged by gel electrophoresis, than did Q758C:GACT (wt) (Figure 2a).
Despite the fact that this variant was extremely novel, it was nonetheless completely functional, and yielded as much or more polymerase expression when paired with its promoter G GTA than did the wild type polymerase paired with the wild type promoter (GACT) (Figure 2b).