Example sentences for: enzyme

How can you use “enzyme” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • , okadaic acid), they were added to the enzyme mixture 10 minutes prior to initiating the reaction with the addition of substrate [ 14 ] .

  • Guinea pig serum samples from animals immunized with 5 μg of enzyme were used to determine the position of linear B-cell epitopes in BPN' Y217L (Figure 7).

  • This created labelled cDNA fragments comprising the most 3' located site of each restriction enzyme, respectively, towards a defined stretch of the poly-A tail of the cDNA, a defined part of the cloning vector and the labelled oligonucleotide.

  • In one report, ALDH1A1 protein was not detected by Western blot, but enzyme activity was detected using flow cytometry and a fluorescent aldehyde substrate [ 5 ] . In a second study, ALDH1A1 protein was detected using an ELISA assay and activity was detected using aldophosphamide as substrate [ 31 ] . In these two studies, designed to look at the relationship of ALDH1A1 activity to antitumor drug resistance, the levels of ALDH1A1 were low in HL-60 compared to other cell types, corresponding to the sensitivity of HL-60 cells to oxazaphosphorines.

  • The molecular mass of the native enzyme was determined by gel filtration using an FPLC-connected Superdex 75 HR 10/30 column (Pharmacia Biotech).


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