Words similar to enzo

Example sentences for: enzo

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Biotin-labeled cRNA was prepared by in vitro transcription (BioArray High-Yield T7, Enzo), and hybridization cocktails were prepared with 20 μg cRNA per sample.
Biotinylated cRNA was prepared using the standard Affymetrix protocol [ 8 ] except that the Epicentre AmpliScribe™ T7 polymerase (Epicentre, Madison, WI) was substituted for the ENZO T7 polymerase (BioArray, High Yield RNA Transcript Labeling Kit, ENZO Diagnostics, Inc., Farmingdale, NY) for 2 of the treated and 2 of the untreated pooled samples.
Biotinylated CTP and UTP (Enzo Biochem Inc., NY) were used together with unlabeled dNTPs in the reaction, and unincorporated nucleotides were removed with RNeasy columns.
Total RNA (20 μg) was used to synthesize double-stranded cDNA using Superscript ®Choice System (Life Technologies), and the template for an in vitro transcription reaction was used to synthesize biotin-labeled antisense cRNA (BioArray™ High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA).
In addition, an antibody against α-actin (Enzo Diagnostics, Inc, Farmingdale, NY) was used to confirm SMCs in the media.