Words similar to egfp-tagged

Example sentences for: egfp-tagged

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Due to the increased molecular weight of the EGFP-tagged cPLA 2 , the two forms of cPLA 2 did not separate as well, making the gel shift more difficult to visualize, but generally mirrored the gel shift characteristics of the endogenous cPLA 2 . These results suggest that, in response to MEK1 inhibition, there is a quantitative, dose-dependent decrease in AA release that correlates well with the loss of ERK activation, but not with the extent of cPLA 2 Ser 505phosphorylation.
Because cPLA 2 is a target of the MEK1/ERK signaling cascade, we assayed the effect of MEK1 inhibition by U0126 on cPLA 2 phosphorylation by analyzing gel shift of cPLA 2 . Phosphorylation of Ser 505results in a retardation of its electrophoretic mobility (gel shift) [ 13 16 ] . In unstimulated cells, EGFP-tagged and endogenous cPLA 2 were nearly completely gel shifted, indicating that most cPLA 2 was phosphorylated on Ser 505(Fig.