Example sentences for: egfp-v

How can you use “egfp-v” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sequencing confirmed that the 13 fluorescent constructs were truncated α s -GFP fusion proteins (with 12 being unique insertions) and that the remaining 22 <EGFP-V> insertions were out of frame (figure 2A).

  • PCR screening for <EGFP-V> insertions within the α

  • PCR screening of 384 Amp r + Kan r resistant colonies identified 44 clones with <EGFP-V> insertions within the α s EE coding region in the correct orientation (11.

  • Insertion sites were identified for all PCR-positive <EGFP-V> transposed clones and all fluorescent / transposed clones by sequencing out of the transposon with a primer complimentary to the EGFP/ECFP coding region (5'-tggccgtttacgtcgccgtcca-3').

  • After transposition and transformation, colonies expressing dual antibiotic resistance were screened with PCR to identify clones in which <EGFP-V> had landed in the correct orientation within the coding region (figure 1C).


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