Example sentences for: egfp-v

How can you use “egfp-v” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Insertion sites were identified for all PCR-positive <EGFP-V> transposed clones and all fluorescent / transposed clones by sequencing out of the transposon with a primer complimentary to the EGFP/ECFP coding region (5'-tggccgtttacgtcgccgtcca-3').

  • PCR and standard subcloning procedures were use to create the initial transposon, <EGFP-V> (full sequence at: http://momotion.med.yale.edu).

  • PCR screening for <EGFP-V> insertions within the α

  • After transposition and transformation, colonies expressing dual antibiotic resistance were screened with PCR to identify clones in which <EGFP-V> had landed in the correct orientation within the coding region (figure 1C).

  • The improved transposons, and , were created in the same way as <EGFP-V>, but Asc I sites were added to facilitate changing the fluorescent protein at a later date (supplemental material).


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