Words similar to egfp-v
Example sentences for: egfp-v
How can you use “egfp-v” in a sentence? Here are some example sentences to help you improve your vocabulary:
Insertion sites were identified for all PCR-positive <EGFP-V> transposed clones and all fluorescent
/ transposed clones by sequencing out of the transposon with a primer complimentary to the EGFP/ECFP coding region (5'-tggccgtttacgtcgccgtcca-3'). PCR and standard subcloning procedures were use to create the initial transposon, <EGFP-V> (full sequence at: http://momotion.med.yale.edu).
PCR screening for <EGFP-V> insertions within the α
After transposition and transformation, colonies expressing dual antibiotic resistance were screened with PCR to identify clones in which <EGFP-V> had landed in the correct orientation within the coding region (figure 1C).
The improved transposons,
and , were created in the same way as <EGFP-V>, but Asc I sites were added to facilitate changing the fluorescent protein at a later date (supplemental material).