Words similar to egfp-cpla

Example sentences for: egfp-cpla

How can you use “egfp-cpla” in a sentence? Here are some example sentences to help you improve your vocabulary:

Stable lines expressing EGFP-cPLA 2 were generated by growing transfected cells in growth medium for 3 d, supplementing the growth medium with 5 mg/ml Geneticin (antibiotic G418-sulfate), and culturing for an additional 2 wk in Geneticin.
However, EGFP-cPLA 2 is functional since it dose-dependently catalyzes release of AA when expressed in cells that lack endogenous cPLA 2 , such as Sf9 cells [ 13 ] and immortalized mouse lung fibroblasts from cPLA 2 α knock-out mice [ 26 ] . In order to enhance AA release in cells containing endogenous cPLA
Stable EGFP-cPLA 2 transfectants were grown on 100 mm dishes at 5 × 10 3cells/cm 2in growth medium for one day, then quiesced in serum-free medium overnight.
EGFP-cPLA 2 was expressed to similar levels as endogenous enzyme but did not contribute significantly to AA release in stably transfected cells.
The protocol for determining AA release is essentially as described [ 15 21 ] . MDCK cells stably expressing EGFP-cPLA 2 were plated in 12-well plates at 5 × 10 3cells/cm 2and incubated in growth medium overnight.