Example sentences for: disrupting

How can you use “disrupting” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Moreover, the 1B4 mutation reveals a mechanism by which U3 gene trap vectors can be expressed without disrupting cellular gene expression and suggests ways to improve the vectors for gene trap mutagenesis.

  • Mutating the conserved leucine to arginine at position 156 is a drastic change, disrupting a γ-branched aliphatic residue with a basic guanidino group, a major chemical and presumably structural substitution.

  • The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.

  • Rab24 contains a unique arginine-rich insert, 126EEDRRRRR 133 [ 25 ] in the domain cognate to loop-8 in H-Ras [ 34 ] . The latter sequence is reminiscent of unusual arginine-rich nuclear localization signals that allow proteins like HIV tet and rev to undergo nuclear translocation by association with importin-β, in the absence of importin-α [ 45 46 ] . Since the loop-8 insert in Rab24 lies adjacent to the conserved N(T)KxD nucleotide binding cassette, we hypothesized that conformational changes caused by the D123I substitution might render the Rab24 mutant capable of disrupting the nuclear import machinery [ 47 ] . To test this possibility, we co-expressed mycRab24wt or mycRab24(D123I) with GR-GFP, a fusion between the glucocorticoid receptor and green fluorescent protein.

  • The T content of concordant and discordant oligonucleotides was similar, with average values of 26% and 29%, respectively, suggesting that UV cross-linking was not preferentially disrupting hybridization specificity of discordant 70-mers.


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