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Example sentences for: disrupting
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The relaxation and decatenation activities of T. maritima topoisomerase I appear to be significantly more efficient than those of the E. coli topoisomerase I [ 33 ] . Based on their primary sequences, a number of bacterial topoisomerase I enzymes do not appear to coordinate any Zn(II) with tetracysteines motifs while other type IA topoisomerase has up to 4 tetracysteine motifs [ 7 ] . The topoisomerase I from Mycobacterium smegmatis has been demonstrated biochemically not to bind Zn(II) [ 34 ] . In contrast, mutation disrupting the fourth Zn(II) motif of Helicobacter pylori topoisomerase I abolished enzyme function in vivo [ 35 ] . Therefore there may be significant differences in the mechanisms of type IA topoisomerases from different organisms with respect to requirement of Zn(II) binding for relaxation activity.
Moreover, the 1B4 mutation reveals a mechanism by which U3 gene trap vectors can be expressed without disrupting cellular gene expression and suggests ways to improve the vectors for gene trap mutagenesis.
Several terrorist operatives were detained by foreign governments, possibly disrupting operations in the Gulf and Italy and perhaps averting attacks against two or three U.S. embassies.
Both forms are toxic to microorganisms, with arsenite disrupting enzyme function, and arsenate behaving as a phosphate analog and interfering with phosphate uptake and utilization [ 1 ] . Microorganisms have evolved a variety of mechanisms for coping with arsenic toxicity, including minimizing the amount of arsenic that enters the cell (e.g.
There are two extreme interpretations of our analysis of the Net1(7m) and Net1 (19m') mutants: First, Cdc5 is not directly involved in disrupting the RENT complex, and second, Cdc5 directly disassembles the RENT complex via phosphorylation of Net1, but because it is not feasible to evaluate all possible 10 9(=2 30) combinations of phospho-site mutants or because the sites phosphorylated by Cdc5 in vivo are different from those in vitro due to the presence of factors such as Sir2, Pol I, and rDNA [ 10 23 ] , we missed key phosphorylation sites that would have given a more definitive phenotype when mutated.
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