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Example sentences for: dhplc

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DHPLC has been developed to screen for DNA variations by separating heteroduplex and homoduplex DNA fragments by ion-pair reverse-phase liquid chromatography [ 22 ] . We used this technique to study eight male patients and one female carrier relative of one of the eight males.
In our study, one likely causative mutation (patient 03 del exn21) was found just by PCR analysis and 5 likely causative mutations were first detected by direct sequencing but each was subsequently detected by DHPLC.
All twelve exons of LMNA were screened by denaturing high performance liquid chromatography (DHPLC) and single strand conformation polymorphism (SSCP) (Figure 4).
Additional file B: Details regarding procedure and cost of DHPLC screening, observations regarding the dystrophin gene, and statistics about DMD carrier females.
The primer set and the sequence of the fragments as well as additional intron sequence are available on the Leiden Muscular Dystrophy pages http://www.dmd.nl/DMD_DHPLC.html.