Words similar to dhplc
Example sentences for: dhplc
How can you use “dhplc” in a sentence? Here are some example sentences to help you improve your vocabulary:
We used a combination of direct sequencing and DHPLC to search for mutations in 8 patients with DMD and a carrier sister.
PCR cycling conditions consisted of an initial denaturation step at 95°C for 15 min. followed by 35 cycles of 94°C for 5 s, the specified annealing temperature (indicated in Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet) for 15 s, 72°C for 30 s and ending with a final elongation step at 72°C for 3 min.
As new mutations are discovered, they could be entered into the database along with any suggested improvements to the DHPLC conditions for a given fragment/alteration.
The DHPLC instrument we used is the WAVE ®system (Transgenomic Inc., Omaha, Ne.)
We calculated the cost of reagents per 50μl PCR and the cost of reagents to run a sample on the DHPLC system and multiplied by the number of samples required to screen a patient.