Words similar to dhplc

Example sentences for: dhplc

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The DHPLC system was obtained halfway into the study and four patients' exons 56 through 78 and the 3'-UTR including exon 79 were analyzed on the DHPLC system prior to sequence analysis.
Direct sequencing of all the fragments is still more costly than DHPLC and our results indicate that initial analysis using the far cheaper DHPLC should precede sequencing thus reducing cost of the analysis.
The unaffected and mixed reactions were then run at pre-determined temperatures (see Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet) on the WAVE ®system and the resultant chromatograms compared for variation in shape or retention time.
Our experience indicates that the multiple different conditions of DHPLC would have detected all of these likely causative mutations and all polymorphisms.
Appropriate melting characteristics are such that variations from unaffected sequence in any portion of the fragment will be detected using ideally only one running temperature but no more than three (see Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet)