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Example sentences for: dhplc

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The primer set and the sequence of the fragments as well as additional intron sequence are available on the Leiden Muscular Dystrophy pages http://www.dmd.nl/DMD_DHPLC.html.
Additional file B: Details regarding procedure and cost of DHPLC screening, observations regarding the dystrophin gene, and statistics about DMD carrier females.
In our study, one likely causative mutation (patient 03 del exn21) was found just by PCR analysis and 5 likely causative mutations were first detected by direct sequencing but each was subsequently detected by DHPLC.
As we found no likely disease-causative mutations in our first pass DHPLC conditions, we sequenced all four patients exons 56-78 and the 7 fragments of the 3'-UTR (the first of which contains exon 79).
Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet