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Example sentences for: dhplc

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Point mutations can be found in both DMD/BMD male patients and asymptomatic or manifesting DMD/BMD carrier females via an effective and convenient process using automated DHPLC screening for variation and direct sequencing for confirmation and analysis at a moderate increase in cost per patient.
Likely disease-causative mutations were found in four of the patients and confirmed in the female relative of patient 01 by direct sequencing of exons 2 through 55, and the three 5'-UTR fragments, the last of which includes the dp427m promoter and exon 1. These were sequenced prior to our obtaining the DHPLC system for screening.
PCR cycling conditions consisted of an initial denaturation step at 95°C for 15 min. followed by 35 cycles of 94°C for 5 s, the specified annealing temperature (indicated in Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet) for 15 s, 72°C for 30 s and ending with a final elongation step at 72°C for 3 min.
Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet
Two variations were detected by DHPLC, both in exon 59, for patients 05 and 06 (Figure 2a) (only patient 06 shown).