Words similar to dhpc
Example sentences for: dhpc
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Proton release and uptake in the aqueous bulk medium were detected from the pR-containing micelles suspended in 1% DHPC, 100 mM NaCl, with 45 μM Cresol Red pH indicator dye.
For the pR samples reconstituted in DHPC, which were used for flash photolysis experiments, the absorbance maxima were similar to those measured in OG (spectra not shown).
Time-resolved UV/vis spectroscopy methods were as described previously [ 18 ] . A Phenylsepharose™-purified pR sample was reconstituted into mixed micelles containing 1,2-diheptanoyl-SN-glycero-3-phosphocholine (DHPC), by adding a 1% solution of the short-chain lipid and then removing most of the detergent on a Sephadex G-25 column equilibrated with 1% DHPC in 100 mM NaCl.
Delipidated bR in octylglucoside is fully capable of M formation and presumably proton release, although with altered kinetics [ 12 16 ] . The requirement for pR to be in lipid to show fast H +release and M formation stems either from a protein/lipid interaction needed to establish a stable, active tertiary structure, or from the need for the phosphate group in DHPC to act as a proton release group.
The latter seems unlikely due to the DHPC molecule being zwitterionic at pH 9.5, with no proton on the trimethyl-modified nitrogen of the choline.