Example sentences for: dh

How can you use “dh” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The autogene pool was transformed into DH5Δlac pLysS cells by electroporation.

  • 1-V5/His (Invitrogen, TA kit), Escherichia coli DH5α cells were transformed, miniprep DNA was prepared using QIAPrep 96 Turbo Miniprep Kit (Qiagen) and subject to sequence analysis with vector-based primers using automated fluorescent sequencer ABI377 (PE Applied Biosytems).

  • To remove non-replicated templates, 2.5 μg of infected cell DNA was digested with Dpn I and precipitated, centrifuged at 14,000 rpm for 10 minutes at 4°C and resuspended in 20 μl dH 2 O. One-fourth of the DpnI -treated DNA was transformed into DH5α cells (recombination-deficient bacterial cells; Invitrogen).

  • A plasmid encoding the HBSAg under the control of the Human Cytomegalovirus Immediate Early gene I (HCMVIE1) enhancer/promoter (pRc/CMV-HBs(S) was obtained from Aldevron (Fargo, ND) [ 20 ] . A plasmid encoding the firefly luciferase gene under the control of the HCMVIE1 enhancer/promoter (pHCMVIE1-luciferase) was used to measure relative gene expression levels following gene transfer by low-volume jet injection, particle bombardment and needle and syringe injection [ 2 ] . Plasmids were propagated in DH5α cells and purified by Bio 101 Merlin Core Services (Carlsbad, CA).

  • E. coli DH5α and E. coli BL21 were grown on LB medium plus kanamycin when transformed with plasmid.


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