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Example sentences for: dh
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For example, the KRC/ZAS-C consensus sequence "ATTTTGTGG" matches completely with 6 nucleotides of the RSS nonamer and 3 flanking nucleotides of the human IgH D1, D2, D3 and D4 gene segments [ 35 ] . Furthermore, the MAST search identified KRC-selected motifs near two chromosomal breakpoints: between a t(11:14) translocation of the Ig DH2-2 gene segment and the B cell lymphoma 1 ( BCL-1 ) gene in a mantle cell lymphoma [ 36 ] , and at a t(11:19) translocation of the myeloid lymphoid leukemia ( MLL ) gene and the ENL/MLLT1/LTG19 gene in a T-cell acute lymphoblastic leukemia [ 37 ] . The other hits were derived from loci of cellular genes, pseduogenes, or DNA fragments [ 41 42 43 44 ] . The expression of those genes has not been shown to be regulated by KRC or other family members, therefore, the biological significance of those MAST results is unknown.
E. coli strain DH5α was used as the host strain for cloning.
The ligations were transformed into DH5α supercompetent cells (Life Technologies) and positive clones were identified by blue-white selection.
After re-precipitation the following day with ethanol, the pellet was resuspended in 10 mM Tris buffer and centrifuged at 200,000 × g for 2 hours at 20°C in the presence of 25 mM MgCl 2 . The final LPS extract was suspended in 10 ml dH 2 O and dialyzed overnight against dH 2 O to remove any residual salt and SDS prior to being lyophilized.
Lipid A extracts were collected and washed three times with 10 ml aliquots of dH 2 O before being lyophilized.
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