Words similar to dh
Example sentences for: dh
How can you use “dh” in a sentence? Here are some example sentences to help you improve your vocabulary:
The autogene pool was transformed into DH5Δlac pLysS cells by electroporation.
DH participated in the statistical analyses and the initial manuscript drafting.
A plasmid encoding the HBSAg under the control of the Human Cytomegalovirus Immediate Early gene I (HCMVIE1) enhancer/promoter (pRc/CMV-HBs(S) was obtained from Aldevron (Fargo, ND) [ 20 ] . A plasmid encoding the firefly luciferase gene under the control of the HCMVIE1 enhancer/promoter (pHCMVIE1-luciferase) was used to measure relative gene expression levels following gene transfer by low-volume jet injection, particle bombardment and needle and syringe injection [ 2 ] . Plasmids were propagated in DH5α cells and purified by Bio 101 Merlin Core Services (Carlsbad, CA).
To remove non-replicated templates, 2.5 μg of infected cell DNA was digested with Dpn I and precipitated, centrifuged at 14,000 rpm for 10 minutes at 4°C and resuspended in 20 μl dH 2 O. One-fourth of the DpnI -treated DNA was transformed into DH5α cells (recombination-deficient bacterial cells; Invitrogen).
The pLD1A15SN:GFP-dynacortin plasmid was transformed into DH1 cells and the cells were grown in Hans' Enriched HL-5 with 7.5 μg/ml G418 [ 30 ] . For experiments with Texas Red-dextran, the fluorescent dextran was electroporated into cells.
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