Example sentences for: dh

How can you use “dh” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The pLD1A15SN:GFP-dynacortin plasmid was transformed into DH1 cells and the cells were grown in Hans' Enriched HL-5 with 7.5 μg/ml G418 [ 30 ] . For experiments with Texas Red-dextran, the fluorescent dextran was electroporated into cells.

  • 1-V5/His (Invitrogen, TA kit), Escherichia coli DH5α cells were transformed, miniprep DNA was prepared using QIAPrep 96 Turbo Miniprep Kit (Qiagen) and subject to sequence analysis with vector-based primers using automated fluorescent sequencer ABI377 (PE Applied Biosytems).

  • DNA was precipitated with ethanol, centrifuged and resuspended in dH 2 O for digestion with restriction enzymes as indicated.

  • Following the secondary PCR amplification, subtracted products from each tester cDNA population were cloned into the PCR IV vector of the TOPO TA cloning kit (Invitrogen, Carlsbad, CA) and used in the transformation of competent DH5α Escherichia coli cells.

  • After heat inactivation of the MuA transposase, 2 μl of the reaction were used to transform 17 μl of DH5α chemically competent cells (Invitrogen) in 96-well format.


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