Example sentences for: di-dc

How can you use “di-dc” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1A) were initially incubated with an pool of 32P-labeled degenerate oligonucleotides and non-specific competitor DNA poly(dI-dC) (10 μg).

  • 1 to 0.5 μg) and an excess non-specific DNA poly(dI-dC) (10 μg).

  • 1 M ETDA, pH 8, 1.7 M sodium acetate, pH 5, 0.1 μg poly-(dI-dC)).

  • Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.

  • The oligonucleotide pool (~500 ng) was incubated with KRC/ZAS-N or KRC/ZAS-C (100 μg each) and 10 μg of non-specific competitor DNA poly(dI-dC).


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