Example sentences for: dgge

How can you use “dgge” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Because the DGGE banding patterns can not be standardized, DGGE data does not represent a permanent record of microbial diversity or biogeography.

  • DGGE: Denaturing Gradient Gel Electrophoresis

  • Existing procedures for detection of point mutations include SSCP (single strand conformational polymorphism) [ 11 12 ] , a variation of SSCP called DOVAM (Detection of Virtually All Mutations) [ 19 ] , Enhanced SSCP [ 28 ] , HA (Heteroduplex Analysis) [ 13 14 ] , PTT (Protein Truncation Test) [ 15 ] , DGGE (Denaturing Gradient Gel Electrophoresis) [ 16 17 18 ] , chemical cleavage of mismatch [ 20 ] , and RNase cleavage [ 21 ] . Each of these procedures has its advantages and limitations but the size of the gene and the cost of the procedures have not made them routine in most laboratories.

  • In fact, to get a permanent record of what microbe each band on the DGGE represents it is necessary to clone and sequence the band.

  • Fortunately, there are other methods for detection of point mutations that can be compared to, or used in addition to, the method presented here, including PTT [ 15 ] , DGGE [ 18 ] , and DOVAM-S [ 19 ] . More information on DGGE analysis of the DMD can be found in the Leiden Muscular Dystrophy web site http://www.dmd.nl/.

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