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Example sentences for: dgge

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Existing procedures for detection of point mutations include SSCP (single strand conformational polymorphism) [ 11 12 ] , a variation of SSCP called DOVAM (Detection of Virtually All Mutations) [ 19 ] , Enhanced SSCP [ 28 ] , HA (Heteroduplex Analysis) [ 13 14 ] , PTT (Protein Truncation Test) [ 15 ] , DGGE (Denaturing Gradient Gel Electrophoresis) [ 16 17 18 ] , chemical cleavage of mismatch [ 20 ] , and RNase cleavage [ 21 ] . Each of these procedures has its advantages and limitations but the size of the gene and the cost of the procedures have not made them routine in most laboratories.
Because the DGGE banding patterns can not be standardized, DGGE data does not represent a permanent record of microbial diversity or biogeography.
DGGE: Denaturing Gradient Gel Electrophoresis
In fact, to get a permanent record of what microbe each band on the DGGE represents it is necessary to clone and sequence the band.
Fortunately, there are other methods for detection of point mutations that can be compared to, or used in addition to, the method presented here, including PTT [ 15 ] , DGGE [ 18 ] , and DOVAM-S [ 19 ] . More information on DGGE analysis of the DMD can be found in the Leiden Muscular Dystrophy web site http://www.dmd.nl/.