Example sentences for: dgt

How can you use “dgt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have been using a map-based cloning strategy to isolate the Dgt gene, previously mapped to the long arm of chromosome 1 [ 8].

  • To identify additional genes within that region by finding microsyntenic regions in Arabidopsis , nucleotide sequences of the three RFLP markers most closely linked to the dgt locus (TG269, TG389, and CT190) were used to identify homeologous sequences in the Arabidopsis genome database [ 18].

  • The approach proved to be less technically complicated than other fingerprinting methods and points to several co-segregating genes for further investigation as potential Dgt candidates.

  • Using restriction-length-fragment polymorphism (RFLP) and RFLP-derived cleaved amplified polymorphic sequence (CAPS) markers (Figure 1) based on previously published tomato genetic and RFLP maps [ 8], initial studies mapped the dgt locus to a region of around 0.8 cM near the bottom of the long arm of tomato chromosome 1. Of 1,308 backcross (BC 1 ) individuals screened, 10 plants were determined to be recombinant between markers TG269 and CT190, whereas no plants were found to be recombinant between TG389 and dgt (Figure 1).

  • Although exceptions to the blanket application of this approach have been noted [ 22], our study successfully applied microsynteny analysis between the tomato and Arabidopsis genomes to facilitate positional cloning of the Dgt gene in tomato.


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