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Example sentences for: degraded
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In identifying targets for therapeutic intervention, it is important to note that the loss of collagen, rather than proteoglycan, is correlated with disease severity [ 6 ] . Furthermore, whereas lost proteoglycan is replaced rapidly, degraded collagen is resynthesized very slowly [ 7 8 ] . Consequently, understanding collagen degradation and providing therapeutics to prevent it are valuable aims.
The other recombination-defective mutants, in otherwise rec +background, did not accumulate the huge linears probably because the DNA was degraded by active RecBCD enzyme or was not produced.
During synthesis of the minus-strand DNA the pregenomic RNA is degraded by the ribonuclease H (RNAseH) activity of P. However, the RNAseH activity does not digest the extreme 5' end of the template RNA, and this ∼ 18 nucleotide capped RNA fragment is then transferred to direct repeat 2 (DR2), where it acts as a primer for synthesis of plus-strand DNA [ 11, 12].
The cDNA:mRNA hybrid was isolated on a Qiaquick PCR purification column (Qiagen) and degraded after addition of 2.5 μl 1 N NaOH for 10 minutes at 37°C.
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
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