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Example sentences for: dbf
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The net1(19m') allele also showed a synthetic growth defect when combined with dbf2-1 . These observations imply that some of the residues mutated in net1(19m') represent in vivo phosphorylation sites that influence the MEN-dependent release of Cdc14 during anaphase.
To evaluate whether the net1(7m) and net1(19m') phosphosite mutants have an impact on FEAR, we analyzed Cdc14 localization in synchronous populations of dbf2-1 , net1(7m) dbf2-1, and net1(19m') dbf2-1 cells following release from an α-factor block in G1 phase at 25°C to α-factor-free medium prewarmed to 37°C (nonpermissive temperature for dbf2-1 ). As shown in Figure 6D, the percentage of cells that completely released Cdc14 during early anaphase (spindle length 0-8 μm) was reduced by ~50% in net1(7m) and ~25% in net1(19m') cells compared with wild-type cells.
The mechanisms by which Cdc5 and Dbf2/Mob1 promote the disassembly of RENT remain unknown, raising the possibility that other Tab proteins serve as intermediaries in this process.
Cdc5 is required for full activation of the Dbf2-Mob1 protein kinase complex [ 15 16 17 ] , and has been proposed to impinge on the MEN both upstream and downstream of Tem1 [ 15 16 17 18 ] . During exit from mitosis, Cdc5 is required for Clb2 destruction, and similar to over-expression of Cdc14 [ 3 ] , induction of Cdc5 triggers precocious Clb2 degradation in pre-anaphase cells [ 19 20 ] . In addition to its role in exit from mitosis, Cdc5 has other functions during mitosis in budding yeast.
First, the sole essential function of the Tem1, Cdc15, Dbf2/Dbf20, and Mob1 (but not Cdc5) proteins are to promote the release of Cdc14 from the nucleolus, as cells lacking these proteins can grow upon simultaneous reduction of NET1 activity.
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