Words similar to dbf
Example sentences for: dbf
How can you use “dbf” in a sentence? Here are some example sentences to help you improve your vocabulary:
, submitted), Mob1 binds an essential protein kinase involved in spindle pole body duplication [ 27 ] , and Tem1, Cdc15, Dbf2, and Mob1 have all been implicated in cytokinesis ( [ 28 29 30 31 ] ; reviewed in [ 1 ] . The tab mutants provide a unique opportunity to address this issue.
Transcription has been postulated to play an important role in triggering exit from mitosis [ 20 ] . For example, Dbf2, a protein kinase that promotes exit from mitosis, is associated with the CCR4 transcription regulatory complex [ 21 ] . In addition, the Swi5 transcription factor activates SIC1 expression during late anaphase, and both sic1Δ and swi5Δ are synthetically lethal with
This explanation is unlikely to account fully for Cdc5's role in exit from mitosis, however, because (1) overexpression of Cdc5, but not Dbf2/Mob1, provoked ectopic release of Cdc14 from the nucleolus; and (2) the Dbf2/Mob1 kinase complex is at least partially activated and is correctly localized to the spindle pole body in 50% of cdc5 cells at the nonpermissive temperature [ 16 17 ] .
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
In S. cerevisiae , disassembly of the RENT complex and the subsequent release of Cdc14 from the nucleolus drive cells to exit mitosis [ 8 9 ] . This event is thought to require two independent signals: an unknown mitosis-specific signal and a signal from the mitotic exit network (MEN) proteins including Tem1, Cdc15, and Dbf2 [ 8 9 ] . The Polo-like kinase Cdc5 also belongs to the MEN, thus we tested whether it too is required for release of Cdc14 from the nucleolus by using indirect immunofluorescence to compare Cdc14 localization patterns in wild type and cdc5-1 cells.
Loading...