Words similar to dbf
Example sentences for: dbf
How can you use “dbf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cdc15, Dbf2, and Mob1 are not properly localized to the spindle pole body in a significant fraction of cdc5-1 cells [ 30 31 17 ] , and Dbf2/Mob1 protein kinase activity is diminished in cdc5 cells [ 15 16 17 ] , suggesting that at least one function of Cdc5 is to generate maximally active Dbf2/Mob1, which can then trigger Cdc14 release.
GAL -driven overexpression of Dbf2/(GST-Mob1) protein kinase complex did not dislodge Cdc14 from the nucleolus (data not shown).
In S. cerevisiae , disassembly of the RENT complex and the subsequent release of Cdc14 from the nucleolus drive cells to exit mitosis [ 8 9 ] . This event is thought to require two independent signals: an unknown mitosis-specific signal and a signal from the mitotic exit network (MEN) proteins including Tem1, Cdc15, and Dbf2 [ 8 9 ] . The Polo-like kinase Cdc5 also belongs to the MEN, thus we tested whether it too is required for release of Cdc14 from the nucleolus by using indirect immunofluorescence to compare Cdc14 localization patterns in wild type and cdc5-1 cells.
The net1(19m') allele also showed a synthetic growth defect when combined with dbf2-1 . These observations imply that some of the residues mutated in net1(19m') represent in vivo phosphorylation sites that influence the MEN-dependent release of Cdc14 during anaphase.
Li, personal communication), Cdc15 [ 28 ] , or Dbf2/GST-Mob1 (RA, unpublished data) had little or no detectable effect on either exit from mitosis or cell proliferation, suggesting that the Tem1/Cdc15/Dbf2/Mob1 branch of the MEN plays a more restricted role in promoting the exit from mitosis.
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