Words similar to dbf
Example sentences for: dbf
How can you use “dbf” in a sentence? Here are some example sentences to help you improve your vocabulary:
They encode the GTP-binding protein Tem1 and its putative guanine nucleotide releasing factor Lte1, the dual specificity protein phosphatase Cdc14, protein kinases Cdc5, Cdc15, and Dbf2/Dbf20, the Dbf2-binding protein Mob1 (reviewed in [ 4 ] ) and the spindle-pole-body component Nud1 [ 5 ] . Conditional-lethal temperature sensitive (ts) mutations in any of the MEN genes cause cells to arrest in late mitosis with elevated mitotic Cdk activity when shifted to the restrictive temperature.
, submitted), Mob1 binds an essential protein kinase involved in spindle pole body duplication [ 27 ] , and Tem1, Cdc15, Dbf2, and Mob1 have all been implicated in cytokinesis ( [ 28 29 30 31 ] ; reviewed in [ 1 ] . The tab mutants provide a unique opportunity to address this issue.
Li, personal communication), Cdc15 [ 28 ] , or Dbf2/GST-Mob1 (RA, unpublished data) had little or no detectable effect on either exit from mitosis or cell proliferation, suggesting that the Tem1/Cdc15/Dbf2/Mob1 branch of the MEN plays a more restricted role in promoting the exit from mitosis.
Tem1 recruits Cdc15 to the spindle pole body, which in turn recruits and phosphorylates the Dbf2/Mob1 protein kinase complex, thereby switching it on [ 2 14 15 16 17 ] . The Tem1-signaling pathway eventually triggers disassembly of the RENT complex, enabling the release of Cdc14 (and a portion of Sir2) from Net1.
The net1(19m') allele also showed a synthetic growth defect when combined with dbf2-1 . These observations imply that some of the residues mutated in net1(19m') represent in vivo phosphorylation sites that influence the MEN-dependent release of Cdc14 during anaphase.
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