Words similar to dbf
Example sentences for: dbf
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To evaluate whether the net1(7m) and net1(19m') phosphosite mutants have an impact on FEAR, we analyzed Cdc14 localization in synchronous populations of dbf2-1 , net1(7m) dbf2-1, and net1(19m') dbf2-1 cells following release from an α-factor block in G1 phase at 25°C to α-factor-free medium prewarmed to 37°C (nonpermissive temperature for dbf2-1 ). As shown in Figure 6D, the percentage of cells that completely released Cdc14 during early anaphase (spindle length 0-8 μm) was reduced by ~50% in net1(7m) and ~25% in net1(19m') cells compared with wild-type cells.
The mechanisms by which Cdc5 and Dbf2/Mob1 promote the disassembly of RENT remain unknown, raising the possibility that other Tab proteins serve as intermediaries in this process.
net1 tab 2-1bypassed cdc15Δ, tem1Δ, dbf2Δ dbf20Δ, mob1Δ, but not cdc14Δ (Figure 4A; [ 15 ] ). In addition, it bypassed cdc5Δ with negligible (1000-10,000 fold lower) efficiency (Fig.
In S. cerevisiae , disassembly of the RENT complex and the subsequent release of Cdc14 from the nucleolus drive cells to exit mitosis [ 8 9 ] . This event is thought to require two independent signals: an unknown mitosis-specific signal and a signal from the mitotic exit network (MEN) proteins including Tem1, Cdc15, and Dbf2 [ 8 9 ] . The Polo-like kinase Cdc5 also belongs to the MEN, thus we tested whether it too is required for release of Cdc14 from the nucleolus by using indirect immunofluorescence to compare Cdc14 localization patterns in wild type and cdc5-1 cells.
This explanation is unlikely to account fully for Cdc5's role in exit from mitosis, however, because (1) overexpression of Cdc5, but not Dbf2/Mob1, provoked ectopic release of Cdc14 from the nucleolus; and (2) the Dbf2/Mob1 kinase complex is at least partially activated and is correctly localized to the spindle pole body in 50% of cdc5 cells at the nonpermissive temperature [ 16 17 ] .
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