Words similar to dbf
Example sentences for: dbf
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First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
This explanation is unlikely to account fully for Cdc5's role in exit from mitosis, however, because (1) overexpression of Cdc5, but not Dbf2/Mob1, provoked ectopic release of Cdc14 from the nucleolus; and (2) the Dbf2/Mob1 kinase complex is at least partially activated and is correctly localized to the spindle pole body in 50% of cdc5 cells at the nonpermissive temperature [ 16 17 ] .
GAL -driven overexpression of Dbf2/(GST-Mob1) protein kinase complex did not dislodge Cdc14 from the nucleolus (data not shown).
dbf2Δ [ 22 7 ] . To address if production of new proteins is essential for exit from mitosis, we examined the effects of the protein synthesis inhibitor cycloheximide on Clb2 degradation, a key aspect of this process.
Cdc5 is required for full activation of the Dbf2-Mob1 protein kinase complex [ 15 16 17 ] , and has been proposed to impinge on the MEN both upstream and downstream of Tem1 [ 15 16 17 18 ] . During exit from mitosis, Cdc5 is required for Clb2 destruction, and similar to over-expression of Cdc14 [ 3 ] , induction of Cdc5 triggers precocious Clb2 degradation in pre-anaphase cells [ 19 20 ] . In addition to its role in exit from mitosis, Cdc5 has other functions during mitosis in budding yeast.
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