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Example sentences for: dbp

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5%) of the ~10,000 genes screened by mRNA differential display and cDNA microarrays in the cortex to date change their expression with sleep deprivation [ 45 ] . It is intriguing and encouraging that, of the initial genes we assayed, three changed their expression with sleep deprivation ( per1 , per2 , dbp ), none of which were identified in the aforementioned screens.
From each animal, quantitative real-time PCR determinations of 5 target genes ( cry1,2, per1,2 , and dbp ) and a reference cDNA (glyceraldehyde-3-phosphate dehydrogenase, g3pdh ) were made from the cortex, basal forebrain, and hypothalamus (primer/probe sequences available upon request).
+ c 9 (SBP) i1 + c 10 (DBP) i1 + c 11 (WT) i1 + u 3i
Messenger RNA levels for per1 , 2 , cry1 , 2 , and dbp were quantified by RT-PCR in three brain areas (cerebral cortex, basal forebrain, and hypothalamus) from C57BL/6 mice that were sacrificed immediately after 6 h of SD or after 4 h of recovery sleep (ZT10).
The high per levels in cry1,2 -/-mice and the low per levels in clock mutant mice [ 26 47 ] correlate with their contrasting sleep phenotype (see above; [ 34 ] ). In this context, the sleep abnormalities in dbp -/-mice might also be related to a reduction in per expression since, at least in vitro , DBP can amplify the CLOCK:BMAL1-induced transcription of per [ 48 ] , but it is not known whether per transcript levels are altered in dbp -/-mice.