Words similar to cyclophilin
Example sentences for: cyclophilin
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The template DNA used for making probes to detect expression of PR-1 , PR-5 and ROC1 (cyclophilin, used to normalize for RNA loading) were PR-1 and PR-5 cDNAs [ 14 ] and an Eco RI fragment of pCG22 [ 34 ] , respectively.
Cyclophilin, a gene known not to alter following axotomy [ 13 ] , was used to correct for loading (Figure 2D).
. One of the blots in each batch was probed for cyclophilin to act as a loading control.
Loading levels between samples on each blot were normalized using the cyclophilin levels from the control blot.
Sample RNA (20μg total RNA) was coprecipitated with the BRCA1 probe and the cyclophilin control probe [ 32], resuspended in Hyb-speed RPA (Ambion) hybridization buffer at 95°C, and then incubated at 68°C for 10min.