Words similar to cy
Example sentences for: cy
How can you use “cy” in a sentence? Here are some example sentences to help you improve your vocabulary:
Visualization of AlexaFluor-647-stained lymphocytes was achieved using a Cy5 filter cube (620 nm excitation, 660 nm long-pass beam splitter dichroic mirror, and 700 nm emission), while AlexaFluor-488-stained lymphocytes were visualized with a fluoroisothiocyanate (FITC) filter cube (480 nm excitation, 505 nm long-pass beam splitter dichroic mirror, and 535 ± 25 nm emission).
To correct for variations in cDNA labeling efficiency, we normalized the Cy5/Cy3 fluorescence ratios for all genes in each array hybridization to obtain an average log 2 (ratio) of 0. We restricted our analysis to genes with measurable expression in 80% of the samples we analyzed.
The following reagents were added: 6 μl of 5X first strand buffer, 3 μl 0.1 M DTT, 0.7 μl 50X dNTP (25 mM dATP, dCTP, dGTP and 10 mM dTTP), 3 μl 1 mM Cy3-dUTP or Cy5-dUTP and 1.7 μl Superscript™ II (200 U/μl).
The B. subtilis control cocktail contained equal proportions (wt/wt) of each RNA species and was spiked into cDNA reactions at 125 pg/2 ug mRNA in order to normalize Cy3 and Cy5 signals after scanning.
The cDNA was purified with ethanol precipitation and resuspended in 5 μl of 2x coupling buffer, then coupled with 5 μl of Cy3 or Cy5 dye for 1 hr in the dark.
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