Words similar to cv-

Example sentences for: cv-

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To assay pRB-directed phosphatase activity, [ 32P]-labeled pRB substrate was prepared by immunoprecipitation with monoclonal antibody to pRB, PMG3-245 (PharMingen), from [ 32P]-labeled CV-1P cells as described previously [ 20 ] . For the source of enzyme, immunocomplexed 6His-HA-PP1α from induced LLWO2F cells were mixed and incubated with [ 32P]-labeled immunoprecipitated pRB for 30 minutes at 30°C as previously described [ 20 ] . Phosphatase reactions were terminated by the addition of SDS-PAGE sample buffer and boiling.
CV-1 cells were infected with the virus inoculum for 1 h; the growth medium was removed and 2.5% FBS in DMEM added to the cells.
Other mechanism by which AR could show an altered response results from an increase in its sensitivity to very low levels of androgen [ 22 23 ] . We tested the role of active Stat3 in this possibility cotransfecting CV-1 cells with an androgen responsive reporter (ARE-TATA-luciferase), AR, in the presence or in the absence of constitutively active Stat3C.
To test whether Stat3 could alter the specificity of AR to other HREs, CV-1 cells were cotransfected with reporter plasmids responsive to androgen (ARE-TATA-luciferase) or to estrogen (ERE-TATA-luciferase), in the presence or absence of AR and constitutively active Stat3C (Fig.
CV-1 cells were cotransfected with a reporter plasmid responsive to androgen (ARE-TATA-luciferase) in the presence or absence of AR and constitutively active Stat3C (Fig.