Words similar to cuvette
Example sentences for: cuvette
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Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
One interesting difference between the cuvette and single cell experiments is the time at which the second phase begins.
These results are consistent with the cuvette studies and directly demonstrate that within a narrow time window after MTX challenge, U73343 can rescue BAECs from oncotic cell death.
The aqueous extract of freeze-dried cat's claw ( Uncaria tomentosa or Uncaria guianensis ), vitamin C or green tea was standardized to give a stock solution (25 mg/ml) and filtered at 20 μm using a Whatman paper N° 4. Aliquots (25 μl) were placed in a cuvette and an ethanolic solution of DPPH (100 μM) was added to a final volume of 1 ml.
Fura-2-loaded BAECs were suspended in HBS, placed in a cuvette at 37°C, and the response to extracellular application of MTX was recorded as a function of time (Fig.
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