Words similar to cuvette
Example sentences for: cuvette
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Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
2and 3reflect the average response of the entire population of BAECs within the cuvette.
Although we do not know the reason for this difference, the population studies were performed on dispersed cells in a cuvette whereas, the single cell studies were performed on BAECs attached to glass coverslips.
One interesting difference between the cuvette and single cell experiments is the time at which the second phase begins.
Each cuvette was pulsed at 200 V, 950 uF using a BioRad Genepuls electroporator.
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