Words similar to cuvette
Example sentences for: cuvette
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The aqueous extract of freeze-dried cat's claw ( Uncaria tomentosa or Uncaria guianensis ), vitamin C or green tea was standardized to give a stock solution (25 mg/ml) and filtered at 20 μm using a Whatman paper N° 4. Aliquots (25 μl) were placed in a cuvette and an ethanolic solution of DPPH (100 μM) was added to a final volume of 1 ml.
The cuvette experiments suggest that BAECs challenged with MTX can be rescued from oncotic cell death if U73343 is added to the bath solution within a narrow time frame after the addition of MTX.
2and 3reflect the average response of the entire population of BAECs within the cuvette.
Although we do not know the reason for this difference, the population studies were performed on dispersed cells in a cuvette whereas, the single cell studies were performed on BAECs attached to glass coverslips.
Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.