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Example sentences for: cleaves
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Human Fhit [ 14 ] and the S. cerevisiae Fhit homolog [ 13 ] , which was called Aph1 but is here termed Hnt2 under nomenclature aproved by the Saccharomyces Genome Database, cleave ApppA more readily while Aph1, the S. pombe homolog, cleaves AppppA more readily [ 20 ] . Consistent with the ApppA hydrolase activity of purified Fhit protein, most cancer cell lines that are Fhit negative at the protein level have higher levels of ApppA than cell lines that are Fhit positive [ 21 ] . Nonetheless, the actual concentrations of dinucleoside polyphosphates were submicromolar in every cell culture sample [ 21 ] and thus, under the reported culture conditions, the measured dinucleoside polyphosphates would not be expected to occupy the Fhit active site substantially [ 19 ] . Dinucleoside polyphosphate levels were measured in adenine-requiring S. cerevisiae strains before or after disruption of the Fhit-homologous HNT2 gene [ 13 ] and in adenine-requiring S. pombe strains as a function of disruption and overexpression of the Fhit-homologous aph1 gene [ 22 ] . Recently, it was observed that diadenosine polyphosphates undergo a divalent cation-dependent conformational change that might mediate their biosynthesis, catabolism or signaling properties [ 23 ] .
This ribonucleoprotein (RNP) complex is an endonuclease, which can initiate reverse splicing into an intron-less target site in a process known as retrohoming [ 3 ] . After the intron RNA reverse splices into one strand of the double-stranded DNA, the intron-encoded protein cleaves the other strand at position +9 or +10 downstream of the intron insertion site, and the generated 3' end of the second strand is used as a primer for reverse transcription of the inserted intron RNA.
The commentariat cleaves the Clinton Scandals into three issues this week: 1) the mud-storm surrounding Kathleen Willey; 2) Congress' readiness to receive Kenneth Starr's report; and 3) Robert Bennett's about-face on publicizing Paula Jones' amorous life.
In this regard, the S. cerevisiae RNase III orthologue Rnt1p not only cleaves the 35 S rRNA precursor within the 3'-ETS [ 27 28 29 ] , but also processes snoRNA and snRNA precursors [ 30 31 32 33 ] . Further biochemical studies are required to identify the RNA targets for mammalian RNase III and to determine its involvement in RNA maturation pathways.
A signal peptidase cleaves the signal sequence and the trimmed protein is shuttled through the Golgi for secretion.
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