Words similar to cleave
Example sentences for: cleave
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The amount of adenoviral genomes per cell was determined by a multiplex real-time polymerase chain reaction [ 22 ] . Real-time PCR is based on the 5'-3' nuclease activity of AmpliTaq Gold polymerase, which allows it to cleave fluorogenic probes resulting in fluorescence.
In this assay, the hammerhead ribozyme was able to cleave about 50% of the M6P/IGF2R target within 2 hr of incubation, and by 12 hrs, more than 90% of the M6P/IGF2R target was converted to the expected products (Fig.
RNAseH cleaves the RNA strand in a RNA:DNA heteroduplex, but it does not cleave DNA or RNA that is not hybridized to DNA.
By considering the stiffness of a D. discoideum cell [ 9 10 ] , we estimate the minimal required force to cleave a cell as a function of the cell's geometry.
Thus, a reduction in human RNase III levels in vivo causes the accumulation of specific rRNA processing intermediates [ 18 ] . Consistent with this functional role, human RNase III localizes to the nucleolus in a cell-cycle-dependent manner [ 18 ] . A truncated form of human RNase III has been purified and shown to cleave dsRNA in vitro [ 18 ] . However, little else is known of the functional roles or the mechanistic features of this enzyme.