Words similar to cephalothin

Example sentences for: cephalothin

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Wild-type L1 exhibited k cat values of 41 ± 1 s -1, 1.9 ± 0.1 s -1, 42 ± 1 s -1, and 82 ± 5 s -1for the cephalosporins, nitrocefin, cefoxitin, cefaclor, and cephalothin.
In studies with cefoxitin, cefaclor, and cephalothin as substrate, the observed k cat values for the S224A and S224K mutants were 2- to 7-fold lower; however, the k cat values when using nitrocefin as substrate were slightly higher (< 2-fold).
When using the cephalosporins, nitrocefin, cefoxitin, and cephalothin, as substrates and the I164A mutant, there were 2- to 10-fold (only for cefoxitin) increases in K m and 2- to 4-fold increases in k cat observed (Table 3).
The changes in molar absorptivities (Δε) used to quantitate products were (in M -1cm -1): nitrocefin, Δε 485 = 17,420; cephalothin, Δε 265 = -8,790; cefoxitin, Δε 265 = -7,000; cefaclor, Δε 280 = -6,410; imipenem, Δε 300 = -9,000; meropenem, Δε 293 = -7,600; biapenem, Δε 293 = -8,630; ampicillin, Δε 235 = -809; and penicillin G, Δε 235 = -936.
In kinetic studies using substrates with low K m values (cefoxitin, nitrocefin, and cephalothin) or with small Δε values (penicillin and ampicillin), we typically used substrate concentrations varied between ~ K m and 10 × K m and used as much of the ΔA versus time data (that was linear) as possible to determine the velocity.