Words similar to ceramide
Example sentences for: ceramide
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Oligonucleosomal DNA fragmentation was used as a biochemical marker of endothelial cell apoptosis in which cells were untreated for 48 h, treated with TNFα (50 ng/ml) for 12, 24 or 48, or treated with C2 ceramide (50 μM) for 12 h.
Changes in ceramide production in response to TNFα treatment were presented as a percentage of the untreated control value.
The essential step in plasma membrane destabilization and MP-release very likely is a caspase-dependent sphingomyelin hydrolysis, which leads to the production of ceramide [ 28 29 30 ] . It is possible, however, that sphingomyelin hydrolysis and MP-release may be a consequence of the action of initiator caspases or other ZVAD sensitive proteases rather than only executioner caspases [ 15 31 32 ] . Our study was performed in HUVEC culture, which may have particular properties in respect of MP release when compared with endothelial cells from other organs.
. Treatment of CLENDO cells with an exogenous source of ceramide (C2, 50 μM) caused a significant decrease in cell viability starting 24 h after treatment compared to untreated cells cultured for 72 h (Fig.
2α (1 μM; Sigma Chemical Company; St. Louis, MO) or C2 ceramide (50 μM; BioMol; Plymouth Meeting, PA) for 24, 48 and 72 h.