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Example sentences for: cca
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By looking at whole-genome angles as well as angles within subspaces of interest, the CCA reveals a wealth of information about the expression vectors being studied (Figure 3).
In the case of the HCV RNA, we panned a sequence (AA UUG CCA GGA CGA CCG GGU CCU UUC UUG GAU CAA CCC GCU CAA UGC CUG GAG AUU) predicted to bind to at least one of the proteins comprising the translation complex eukaryotic Initiation Factor 3 (eIF3; [ 11 12 13 14 ] ). Published reports identify the p120 subunit of eIF3 as the one binding to the apical loop of the domain III of HCV 5'UTR, and p170 (also called p160) as binding the stem portion of the domain III.
This overall depiction is called a colorimetric comparison array (CCA).
Polymerization of ribonucleoside triphosphates (NTPs), which is central to a variety of crucial biological processes, including transcription, primer synthesis during DNA replication, addition of polyA tails to mRNA, addition of CCA to tRNA, uridylation in RNA editing, replication of RNA viruses, amplification of RNA in eukaryotic post-transcriptional gene silencing (PTGS), and oligoadenylate synthesis during interferon signaling, is catalyzed by a broad variety of distinct enzymes [ 1 2 3 4 ] . These polymerases belong to two major mechanistic categories, those that copy a nucleic acid template and those that are independent of a template.
The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
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