Words similar to ccaat
Example sentences for: ccaat
How can you use “ccaat” in a sentence? Here are some example sentences to help you improve your vocabulary:
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
We investigated three potential transcription factor binding sites (CCAAT, κE2, and Ets) within the 43 bp regulatory promoter region and showed that only the Ets binding site influences CD5 expression in EL4 T cells.
In some differentiating cell types, changes in both gene transcription and proliferation are regulated by the same transcription factor [ 1 2 3 4 5 6 7 8 9 ] . CCAAT/Enhancer Binding Protein alpha (C/EBPα) is a transcription factor that is required for the differentiation of a number of tissues [ 10 11 12 13 14 15 16 17 18 ] . Mice homozygous for C/EBPα null alleles have severe defects in tissues involved in metabolic homeostasis [ 19 20 21 ] . Cellular proliferation is elevated in the liver of these knockout mice [ 22 ] suggesting that C/EBPα blocks proliferation in vivo.
5b, lane 4) and CCAAT mutant sequence (not shown) did not compete for binding.
Taken together, these results indicate that substitution of adenine at position 3 in the DQA1 Y box for guanine in the consensus Y-box sequence significantly strengthens DEK binding, as do bases at positions 5 and 6 in the reverse CCAAT sequence.
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