Words similar to ccaat

Example sentences for: ccaat

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Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
The consensus ( HLA-DRA ) Y-box sequence differs from the HLA-DQA1*0101 Y box by a single nucleotide immediately 5' of the reverse CCAAT pentamer; this single-nucleotide polymorphism significantly changes the electrophoretic pattern (Fig.
5b, lane 4) and CCAAT mutant sequence (not shown) did not compete for binding.
As figure 2shows, mutations in the CCAAT or κE2 binding site resulted in minor reductions in reporter activity.
The mutagenesis primers corresponding to each of the five regions, Ets (mutE), κE2 (mutK), CCAAT (mutC), CD5X (Xmut1, 2, and 3), and CD5Y (Ymut1 and 2) were synthesized at the Protein and Nucleic Acid Facility, Stanford University.