Words similar to ccaat
Example sentences for: ccaat
How can you use “ccaat” in a sentence? Here are some example sentences to help you improve your vocabulary:
In some differentiating cell types, changes in both gene transcription and proliferation are regulated by the same transcription factor [ 1 2 3 4 5 6 7 8 9 ] . CCAAT/Enhancer Binding Protein alpha (C/EBPα) is a transcription factor that is required for the differentiation of a number of tissues [ 10 11 12 13 14 15 16 17 18 ] . Mice homozygous for C/EBPα null alleles have severe defects in tissues involved in metabolic homeostasis [ 19 20 21 ] . Cellular proliferation is elevated in the liver of these knockout mice [ 22 ] suggesting that C/EBPα blocks proliferation in vivo.
Binding reactions were carried out at room temperature for 30 min in a total volume of 15 μl and contained 2-5 μg of nuclear extracts, 5 μl of 5X gel shift binding buffer (20% glycerol, 5 mM MgCl 2 , 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris-HCl, pH 7.5), 2 μg poly (dI-dC) and 3 × 10 4cpm/μl of [ 32P] labeled CREB (5'-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3'), NF-κB (5'-AGT TGA GGG GAC TTT CCC AGG C-3') (Promega Gel Shift Assay Systems, Madison, WI, USA) or CCAAT/enhancer binding protein (C/EBP) (5'-TGC AGA TTG CGC AAT CTG CA-3') (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) oligonucleotides.
C/EBPα CCAAT/Enhancer Binding Protein alpha
The consensus ( HLA-DRA ) Y-box sequence differs from the HLA-DQA1*0101 Y box by a single nucleotide immediately 5' of the reverse CCAAT pentamer; this single-nucleotide polymorphism significantly changes the electrophoretic pattern (Fig.
NF-Y binding to the MHC class II gene Y box anchors a complex assembly of nuclear proteins that occupies several regulatory elements over a great distance [ 20 21 22 ] . In the DQA1 promoter Y box, a reverse CCAAT motif with a partially overlapping TG-rich sequence shares sequence identity with the HIV-2 DEK-binding site at 7 of 10 positions (Fig.
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