Example sentences for: calbiochem

How can you use “calbiochem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Actinomycin D, roscovitine, phorbol ester and lipopolysaccharide (LPS) were purchased from Calbiochem.

  • For pulse-labeling, the cells were washed three times with DMEM lacking Met and Cys and then incubated in the same medium containing 80 μCi/ml of 35S-labeled Met and Cys (Tran 35S-label, ICN) and the proteasome inhibitor MG-132 (10 μM, Calbiochem) for 1.5 hours.

  • The levels of sulfated GAGs in lavage fluids were determined using a colorimetric dye-binding assay using 1,9-dimethylmethylene blue [ 23 ] . The levels of total nitrite in lavage fluids were measured with Nitric Oxide Assay kits (Calbiochem ®; Biosciences Inc, La Jolla, CA, USA).

  • The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).

  • AG126 and PD98059 were purchased from Calbiochem (San Diego, CA).


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