Words similar to calbiochem
Example sentences for: calbiochem
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Tumor necrosis factor α (TNFα) protease inhibitor 2 and Y-27632 were purchased from Calbiochem (San Diego, California, United States).
Sections were processed for antibody staining using the following antibodies: rabbit [ 86 ] and sheep (BioDesign) anti-SOD1 (both recognize mouse and human SOD1), mouse anti-cytochrome c oxidase subunit CO1 and CO4 (Molecular Probes, OR), rabbit anti-TOM20 and TOM40 (Santa Cruz Biotechnology, CA), mouse anti-cytochrome c (BD PharMingen, San Diego, CA), sheep anti-catalase (The Binding Site, Birmingham, England), rabbit anti-catalase (Calbiochem, SanDiego, CA), rabbit anti-PMP70 (ABR, Golden, CO or Axxora, SanDiego, CA), and rabbit anti-cathepsin D (DAKO, Carpinteria, CA) according to the protocol described previously [ 26 28 ] . The stained sections were examined and digitized using a confocal microscope (Leica TCS-SP).
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
Porcine insulin was purchased from Calbiochem (San Diego, CA, USA), wortmannin, LY294002, and rapamycin were purchased from Sigma-Aldrich Company (St.
UCB (Calbiochem) was purified by alkaline extraction of a chloroform solution, recrystallized twice from chloroform-methanol [ 30 ] , dried under Argon, stored in
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