Words similar to calbiochem

Example sentences for: calbiochem

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Eluted protein was concentrated by dialysis against powdered polyethylene glycol (Aquacide; Calbiochem), then dialyzed into 20% glycerol, 20 mM Tris pH 7.5, 20 mM KCl, 1 mM EDTA, 1 mM DTT.
Cellular 4-HNE protein adducts were detected by rabbit anti-4-HNE antibodies (Calbiochem, San Diego, CA, used at 1:250) followed by anti-rabbit secondary antibody labeled with FITC (Dako Corporation, Carpinteria, CA).
The fusion protein was then cleaved with thrombin (Calbiochem, San Diego, CA) to yield the actin-binding domain of α-actinin and the GST carrier.
The two plasmids used encoded either a 1.6 Kb β-galactosidase gene sequence from E. coli or the enhanced green fluorescence protein (EGFP) gene (Calbiochem, Palo Alto), and were purified by the CsCl method.
The primary antibodies used in this set of experiments were from Santa Cruz (anti-ERK 1/2, used at 1:5000; anti-phospho-ERK, used at 1:1000; anti-PPARγ, used at 1:2000) or from Calbiochem (anti-4-HNE, used at 1:2,000).