Words similar to calbiochem
Example sentences for: calbiochem
How can you use “calbiochem” in a sentence? Here are some example sentences to help you improve your vocabulary:
Actinomycin D, roscovitine, phorbol ester and lipopolysaccharide (LPS) were purchased from Calbiochem.
For pulse-labeling, the cells were washed three times with DMEM lacking Met and Cys and then incubated in the same medium containing 80 μCi/ml of 35S-labeled Met and Cys (Tran 35S-label, ICN) and the proteasome inhibitor MG-132 (10 μM, Calbiochem) for 1.5 hours.
The levels of sulfated GAGs in lavage fluids were determined using a colorimetric dye-binding assay using 1,9-dimethylmethylene blue [ 23 ] . The levels of total nitrite in lavage fluids were measured with Nitric Oxide Assay kits (Calbiochem ®; Biosciences Inc, La Jolla, CA, USA).
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
AG126 and PD98059 were purchased from Calbiochem (San Diego, CA).
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