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Example sentences for: calabasas

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A weakly immunogenic lung cancer, Lewis lung carcinoma (3LL, H-2 b) obtained from ATCC (CRL-1642, LLC) was utilized for assessment of cytokines important for SLC/CCL21 - mediated anti-tumor responses in vivo . The cells were routinely cultured as monolayers in 25 cm 3tissue culture flasks containing RPMI 1640 medium (Irvine Scientific, Santa Anna, CA) supplemented with 10% fetal bovine serum (FBS) (Gemini Bioproducts, Calabasas, CA), penicillin (100 U/ml), streptomycin (0.
Complete methods for the stable transfection of HEK-293 cells to express the wild type receptor can be found in a previous report [ 13 ] . Both cell lines were seeded in 6-well dishes at an initial cell density of 1 × 10 6cells/well and were maintained in Dulbecco's modified Eagle's medium (DMEM; GibcoBRL) supplemented with 2 mM L-glutamine, 5% fetal bovine serum (FBS) (Gemini Bio-Products, Calabasas, CA), and 100 μg/ml gentamycin sulfate (Gemini Bio-Products, Calabasas, CA).
Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.