Example sentences for: calcineurin

How can you use “calcineurin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2000), calcineurin (Chin et al.

  • After purification we treated the four proteins with a combination of calcineurin and calmodulin to remove phosphates attached to serines and threonines on the recombinant proteins.

  • Recombinant HA-tagged NFATp proteins (approximately 500 ng) were incubated with calcineurin (500 ng, Sigma) and calmodulin (200 ng, Sigma) in 21 μl of buffer that had the following final concentrations of components: 7 mM Tris-HCl, 5 mM Hepes (pH 7.6), 90 mM NaCl, 15 mM betamercaptoethanol, 0.3 mM DTT, 1 mM EDTA, 30% (v/v) glycerol, 3 mM MgCl 2 , and 1.5 mM MnCl 2 . Control reactions were performed with NFATp proteins in the absence of added calcineurin and calmodulin.

  • Calcineurin and NFAT proteins are abundant in skeletal myofibers, and several lines of evidence support the viewpoint that the calcineurin–NFAT pathway plays a role in mediating activity-dependent gene regulation in muscle [25,26,27,28,29,30,31,32,33,34,35,36,37,38,39].

  • Moreover, when HA-NFATp(331-722), which was not highly phosphorylated, was tested in the nuclei-association assay it was primarily found in the pellet with the nuclei, regardless of whether it was treated with calcineurin or not (data not shown).


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